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A Moonshot for Coral Breeding Was Successful – Hakai Magazine

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Wearing a navy-blue polo neck emblazoned with the Florida Aquarium logo, Keri O’Neil hugs a white cooler at Miami International Airport. “Coral babieeeeees,” she says, before letting out a short laugh. Relief. The container holds 10 plastic bottles teeming with thousands of tiny peach-colored specks. Shaped like cornflakes and no more than a millimeter in length, they are the larvae of elkhorn coral, an endangered species that is as characteristic to the reefs of the Florida Keys and the Caribbean as polar bears are to the Arctic or giant sequoias to Sierra Nevada.

With the larvae kept at 27 °C inside their insulated cooler nestled in the trunk of her car, O’Neil drives back to the Florida Aquarium in Tampa, where she works as senior coral scientist at the aquarium’s Center for Conservation. Once there, the larvae begin their metamorphosis from free-swimming specks into settled polyps, the beginnings of those branching, antler-like shapes that define this species. O’Neil and her colleagues provide everything the coral needs for a strong start in life: warm water with a gentle flow, symbiotic algae that find a home inside the coral’s cells, a soft glow of sunlight, and some ceramic squares “seasoned with algae” that act as landing pads for the larvae.

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This time-lapse video shows the development of elkhorn coral embryos in the lab. Video by Kristen Marhaver

The transformation of larvae into polyps was the final step in a coral breeding project that began on the shores of Curaçao, an island off the coast of Venezuela, in the summer of 2018 and involved a cadre of conservationists and scientists who each specialize in one specific stage of coral development. From collection of eggs during mass spawning events to the cryopreservation of sperm, and from fertilization to larval growth, every step had to go swimmingly for the project to have any chance of success. “It’s like the most stressful relay on Earth,” says Kristen Marhaver, a coral scientist at the Caribbean Research and Management of Biodiversity Foundation in Curaçao, who helped start this relay race by collecting eggs during a nighttime dive at a reef that’s a 45-minute drive from her laboratory. As O’Neil was picking up her coral “babies” in Miami, a second team of scientists at Mote Marine Laboratory and Aquarium in Sarasota, Florida, received its own. The pressure on both labs was immense. To fail now would be to drop the baton just before the final straight.

But, if anything, their efforts were too successful; hundreds of larvae settled as translucent and fragile blobs of tissue (each a single polyp) and then started to divide, branching into the clear waters of their shallow, open-top tanks. Elkhorn coral grows an average of five to 10 centimeters per year, a bamboo-like pace for corals in general. To stop them becoming entangled, O’Neil had to cut, separate, and move her colonies to different paddle pool–sized tanks over the course of the next year. “We almost ended up with a six-foot-by-four-foot [1.8-meter-by-1.2-meter] solid piece of elkhorn coral made up of 400 different individuals,” she says. “They were just outgrowing the tanks.”

A juvenile elkhorn coral colony approximately six months old gets its start in a lab at the Florida Aquarium in Tampa, Florida. The colony’s eggs came from coral in Curaçao and its sperm from coral elsewhere in the Caribbean—coral populations that, under normal circumstances, would not normally have mixed in the wild. Photo by Kristen Marhaver

The rows of coral in O’Neil’s tanks are a window into a former world. The reefs of the Florida Keys were once dominated by elkhorn coral. Visiting these islands that curl southward from Florida like the tip of a bird of prey’s beak, biologist, conservationist, and writer—most notably of Silent Spring, but also of several books on the ocean—Rachel Carson peered into the shallows using a “water glass,” an instrument akin to a glass-bottom bucket. Through this simple portal, she saw great stands of “trees of stone,” a forest of coral. Today, after decades of disease, coastal development, and bleaching, over 95 percent of the state’s elkhorn coral have been lost. And this population isn’t just depleted in number, like a forest that’s been felled, but is also impoverished from within. Some reefs in the Keys descend from a single individual that has reproduced via fragmentation—bits break off the parent coral and start a new colony. This mode of reproduction allows corals to spread, but without the genetic mixing that comes with sex, these clones are more susceptible to disturbances such as disease. The coral larvae raised by O’Neil at the Florida Aquarium are different; they are the product of sperm and egg, a shuffling of genes, and the growth of genetically unique clumps of coral. Reintroducing them could provide a boost to the coral’s genetic diversity—a quick stir to the gene pool—and could save a denuded ecosystem. Their reintroduction could also spell its doom.

Hidden inside the genetic code of the Florida Aquarium’s coral is a map of an atypical origin: the eggs collected from Curaçao were fertilized using sperm from the Caribbean, including Florida. Although the same species (Acropora palmata), these coral populations would never breed in the wild. The distance between the two is hundreds of kilometers and contains the island blockade of the Greater Antilles—an impossible journey for any sperm. The coral housed in the Florida Aquarium are the products of human hands, the latest addition to a recent—and often controversial—trend in conservation known as “assisted gene flow,” shuttling existing genetic diversity to new places.

Elkhorn coral spawn only once a year, triggered by the full moon, but estimating the exact time and date of the spawn is tricky. Scientists in Curaçao dove for more than 40 nights before the elkhorn coral they were monitoring finally released their eggs. Photo courtesy of Smithsonian’s National Zoo

No hands have offered more assistance to these coral than those of Mary Hagedorn, senior research scientist at the Smithsonian Conservation Biology Institute, who is based at the University of Hawai‘i at Mānoa. Hagedorn flew to the Caribbean to guide this project from start to finish. It is her research that made this work possible. Since 2004, she has developed cryopreservation techniques that can freeze coral sperm and—just as importantly—keep them fertile upon thawing. Although cryopreservation has been used for IVF in humans and other mammals for decades, it’s only in the last few years that other coral conservationists have adopted Hagedorn’s techniques for coral sperm. At a time when these methodologies are most needed, Hagedorn’s work has matured into a solid science, says Tom Moore, a coral restoration manager at the National Oceanic and Atmospheric Administration at the time of this project and now in the private sector. “I think we’re going to start seeing a lot more of this done in the course of the next few years.”

Without the option to freeze sperm, coral conservationists have been forced to work within the few hours these sex cells remain viable. In Florida, Moore says, scientists from the Lower Keys would drive north to meet colleagues from the Upper Keys and swap sperm samples on the side of the road, fertilizing eggs there and then before the sperm stopped swimming. With the option to freeze sperm using liquid nitrogen, however, samples can be transported long distances—from Florida to Curaçao, for example. Then, when eggs are collected from the reef, the sperm can be thawed and used in concentrations that make fertilization most likely. Hagedorn’s work opens up new possibilities that, just a few years ago, were largely ignored.

Biologists Kendall Fitzgerald, left, and Claire Lager, of the Smithsonian Conservation Biology Institute, work in the lab where cryopreservation techniques are used to conserve coral as part of a global coral biorepository. Photo courtesy of Smithsonian’s National Zoo

Self-funded for many years, Hagedorn’s research was nearly stopped altogether in December 2011. Her savings had run out and funders didn’t seem to see the potential of her work. “I was a month away from closing my lab,” she says. Then she received an unexpected call from the Roddenberry Foundation, a philanthropic organization set up in memory of Gene Roddenberry, the writer of Star Trek. Since Hagedorn’s work fit the criteria for bold and unique science, the foundation wanted to fund her research for five years. Since then, her work has grown to include frozen larvae, frozen coral symbiotic algae, and frozen coral fragments, and it has been adopted by labs around the world. To help her cryopreservation methods spread, Hagedorn runs workshops and shares her techniques freely; the instructions to build her equipment can be downloaded and then manufactured with a 3D printer.

As with IVF in humans, coral fertilization is not a perfect science. In a study published in 2017, Hagedorn and her colleagues showed that fertilization rates from frozen coral sperm are significantly lower than from fresh sperm, roughly 50 percent versus over 90 percent. And these figures were based on coral that lived as neighbors on the same reef. The researchers wanted to increase genetic diversity in the future (through assisted gene flow), but it was still unknown whether populations that had been isolated for thousands of years could produce viable offspring, especially after their sperm had been frozen. The idea to breed elkhorn coral from the Florida Keys with those from Curaçao was the most extreme test yet of Hagedorn’s methods. It was a moonshot for coral conservation, says O’Neil. “We wanted to do something that had never been done before.”

Mary Hagedorn, senior research scientist at the Smithsonian Conservation Biology Institute, has pioneered coral cryopreservation techniques since 2004. Photo courtesy of Smithsonian’s National Zoo

Marhaver thought that they had a five to 10 percent chance of success. To have hundreds of healthy coral now sitting in tanks barely crossed her mind. Conservationists are more attuned to the vibrations of endangerment, extinction, and loss. To have a moonshot succeed is unfamiliar territory. With the impossible now possible, the next hurdle is moving from the lab to the ocean, a leap that not everyone is comfortable with.

As in medical practice, the first rule of restoring ailing ecosystems is primum non nocere, “first, do no harm.” And what concerns Lisa Gregg, program and policy coordinator at the Florida Fish and Wildlife Conservation Commission (FWC), the organization that decides the fate of the Florida-Curaçao coral, is that they aren’t suited to the local conditions of the Florida Keys, a place that Carson referred to as having an atmosphere that is “strongly and peculiarly [its] own.” These islands are formed from sedimentation, while those of Curaçao and the eastern Caribbean are founded on volcanic activity. Plus, the Florida Keys also have their own unique combination of problems, from infectious disease to coastal development, and from hurricanes to coral bleaching. “We have a lot of problems here,” says O’Neil. “And it is quite likely that the corals that are still alive in Florida after everything that’s happened to them are probably the ones that are best suited to living in Florida and providing offspring that may be capable of surviving in Florida.” If Curaçao genes were introduced, they might lead to lower rates of reproduction, shorter life spans, or lowered resistance to local diseases. Imperceptible at first, such “outbreeding depression” can slowly weaken a population, generation by generation. To introduce genes that haven’t experienced the same history could be a ratchet toward extinction.

The risk of such outbreeding depression is very low, however—a doomsday forecast for Florida’s reefs, many conservationists think. “I’m not so concerned that there’s a huge risk of the Curaçao [genes] causing a major detriment to the native Florida population,” says Iliana Baums, head of marine conservation and restoration at the University of Oldenburg, Germany, who has studied elkhorn coral since 1998. “But that’s based on my knowledge of the literature for other species and modeling and so on. I don’t have any direct evidence for that.” Direct evidence would require reintroduction, a catch-22 of conservation; the very thing that is controversial and potentially dangerous is also the route to understanding.

Elkhorn coral was once one of the most prolific coral species in the Caribbean and Florida Keys. Raising it in the lab could help boost the species, but since the new colonies are derived from eggs and sperm that would not mix under normal circumstances, their release into the wild is stalled. Photo courtesy of Smithsonian’s National Zoo

Gregg was clear with O’Neil, Marhaver, Hagedorn, and their colleagues from the beginning of this project. “They knew right off the bat … that they were not going to be able to out-plant [the coral]. It was never in question.” The FWC has a “nearest neighbor” policy when it comes to conserving Florida’s coral reefs, she says. “With Acropora palmata, I believe the nearest neighbor would be Cuba or Belize. But other acceptable areas to bring corals in from would be Mexico or the Bahamas. If you’ve got corals coming from Curaçao, that’s leaps and bounds away from Florida.”

After nearly 20 years of research and the near closure of her lab, Hagedorn is tired of waiting. She is sympathetic to the FWC’s approach, but also believes that this large population of captive coral should be introduced—in “a restricted and monitored fashion”—given the critical status of A. palmata. “There’s so little coral in Florida now, it’s just a joke,” she says. In addition to tracking their precipitous decline, scientists have tried to find evidence that new, sexually produced elkhorn coral are settling in the area, but they regularly come back empty-handed. Since this species releases sperm and eggs en masse once a year, the lack of natural recruitment is a worrying sign that such mass spawning events are failing. Warmer waters, pollution, a thick covering of algae, and the rarity of mature coral all add up to prevent new baby coral from settling. Whatever the case, successful sexual reproduction—the fertilization of egg and sperm to create a swimming larva—is so low that it no longer supports this population. “Every year, we seem to lose more [coral] without making more, because sexual reproduction isn’t working,” says Baums. “None of us could’ve imagined that these coral populations would die out this fast. I don’t think any one of us could have really wrapped our heads around that, even 10 years ago … I think we’re at the stage that we need to try something new.”

Even with this precipitous decline, there is still time to try a less extreme version of assisted gene flow, O’Neil says. Now that the Florida-Curaçao experiment has been a success, her team can consider crossing coral from Mexico, the Bahamas, or Cuba—just a relative stone’s throw away—with Florida stock. These populations are able to mix naturally: although sperm can’t survive the journey, the planktonic larvae can travel the current from the Bahamas to Florida so are considered part of the same subpopulation. Gregg says that she would support any elkhorn restoration project that conforms to the FWC “nearest neighbor” policy. Until then, such assisted gene flow will remain limited to laboratories and aquariums.

In December 2021, O’Neil said goodbye to the coral she had raised from peach-colored larvae to hand-sized elkhorn recruits. With the project’s end, they were being transported from the Florida Aquarium to the Mote Marine Laboratory and Aquarium, where they joined the rest of the coral grown as part of this study. Some are being exposed to warmer temperatures to see if they are better able to survive in the warmer waters predicted for the future. Others will be transported to museums and aquariums around the United States. The rest sit patiently and continue to divide, to grow, polyp by polyp. They may never be introduced into the wild, but their mere existence opens a wide-angle vista for coral conservation. If such disparate populations can be crossed and grown by the hundred, almost anything is possible. The next coral babies that O’Neil collects from the airport will have simply traveled a shorter distance in their cooler.

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Health Canada approves updated Moderna COVID-19 vaccine

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TORONTO – Health Canada has authorized Moderna’s updated COVID-19 vaccine that protects against currently circulating variants of the virus.

The mRNA vaccine, called Spikevax, has been reformulated to target the KP.2 subvariant of Omicron.

It will replace the previous version of the vaccine that was released a year ago, which targeted the XBB.1.5 subvariant of Omicron.

Health Canada recently asked provinces and territories to get rid of their older COVID-19 vaccines to ensure the most current vaccine will be used during this fall’s respiratory virus season.

Health Canada is also reviewing two other updated COVID-19 vaccines but has not yet authorized them.

They are Pfizer’s Comirnaty, which is also an mRNA vaccine, as well as Novavax’s protein-based vaccine.

This report by The Canadian Press was first published Sept. 17, 2024.

Canadian Press health coverage receives support through a partnership with the Canadian Medical Association. CP is solely responsible for this content.

The Canadian Press. All rights reserved.

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These people say they got listeria after drinking recalled plant-based milks

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TORONTO – Sanniah Jabeen holds a sonogram of the unborn baby she lost after contracting listeria last December. Beneath, it says “love at first sight.”

Jabeen says she believes she and her baby were poisoned by a listeria outbreak linked to some plant-based milks and wants answers. An investigation continues into the recall declared July 8 of several Silk and Great Value plant-based beverages.

“I don’t even have the words. I’m still processing that,” Jabeen says of her loss. She was 18 weeks pregnant when she went into preterm labour.

The first infection linked to the recall was traced back to August 2023. One year later on Aug. 12, 2024, the Public Health Agency of Canada said three people had died and 20 were infected.

The number of cases is likely much higher, says Lawrence Goodridge, Canada Research Chair in foodborne pathogen dynamics at the University of Guelph: “For every person known, generally speaking, there’s typically 20 to 25 or maybe 30 people that are unknown.”

The case count has remained unchanged over the last month, but the Public Health Agency of Canada says it won’t declare the outbreak over until early October because of listeria’s 70-day incubation period and the reporting delays that accompany it.

Danone Canada’s head of communications said in an email Wednesday that the company is still investigating the “root cause” of the outbreak, which has been linked to a production line at a Pickering, Ont., packaging facility.

Pregnant people, adults over 60, and those with weakened immune systems are most at risk of becoming sick with severe listeriosis. If the infection spreads to an unborn baby, Health Canada says it can cause miscarriage, stillbirth, premature birth or life-threatening illness in a newborn.

The Canadian Press spoke to 10 people, from the parents of a toddler to an 89-year-old senior, who say they became sick with listeria after drinking from cartons of plant-based milk stamped with the recalled product code. Here’s a look at some of their experiences.

Sanniah Jabeen, 32, Toronto

Jabeen says she regularly drank Silk oat and almond milk in smoothies while pregnant, and began vomiting seven times a day and shivering at night in December 2023. She had “the worst headache of (her) life” when she went to the emergency room on Dec. 15.

“I just wasn’t functioning like a normal human being,” Jabeen says.

Told she was dehydrated, Jabeen was given fluids and a blood test and sent home. Four days later, she returned to hospital.

“They told me that since you’re 18 weeks, there’s nothing you can do to save your baby,” says Jabeen, who moved to Toronto from Pakistan five years ago.

Jabeen later learned she had listeriosis and an autopsy revealed her baby was infected, too.

“It broke my heart to read that report because I was just imagining my baby drinking poisoned amniotic fluid inside of me. The womb is a place where your baby is supposed to be the safest,” Jabeen said.

Jabeen’s case is likely not included in PHAC’s count. Jabeen says she was called by Health Canada and asked what dairy and fresh produce she ate – foods more commonly associated with listeria – but not asked about plant-based beverages.

She’s pregnant again, and is due in several months. At first, she was scared to eat, not knowing what caused the infection during her last pregnancy.

“Ever since I learned about the almond, oat milk situation, I’ve been feeling a bit better knowing that it wasn’t something that I did. It was something else that caused it. It wasn’t my fault,” Jabeen said.

She’s since joined a proposed class action lawsuit launched by LPC Avocates against the manufacturers and sellers of Silk and Great Value plant-based beverages. The lawsuit has not yet been certified by a judge.

Natalie Grant and her seven year-old daughter, Bowmanville, Ont.

Natalie Grant says she was in a hospital waiting room when she saw a television news report about the recall. She wondered if the dark chocolate almond milk her daughter drank daily was contaminated.

She had brought the girl to hospital because she was vomiting every half hour, constantly on the toilet with diarrhea, and had severe pain in her abdomen.

“I’m definitely thinking that this is a pretty solid chance that she’s got listeria at this point because I knew she had all the symptoms,” Grant says of seeing the news report.

Once her daughter could hold fluids, they went home and Grant cross-checked the recalled product code – 7825 – with the one on her carton. They matched.

“I called the emerg and I said I’m pretty confident she’s been exposed,” Grant said. She was told to return to the hospital if her daughter’s symptoms worsened. An hour and a half later, her fever spiked, the vomiting returned, her face flushed and her energy plummeted.

Grant says they were sent to a hospital in Ajax, Ont. and stayed two weeks while her daughter received antibiotics four times a day until she was discharged July 23.

“Knowing that my little one was just so affected and how it affected us as a family alone, there’s a bitterness left behind,” Grant said. She’s also joined the proposed class action.

Thelma Feldman, 89, Toronto

Thelma Feldman says she regularly taught yoga to friends in her condo building before getting sickened by listeria on July 2. Now, she has a walker and her body aches. She has headaches and digestive problems.

“I’m kind of depressed,” she says.

“It’s caused me a lot of physical and emotional pain.”

Much of the early days of her illness are a blur. She knows she boarded an ambulance with profuse diarrhea on July 2 and spent five days at North York General Hospital. Afterwards, she remembers Health Canada officials entering her apartment and removing Silk almond milk from her fridge, and volunteers from a community organization giving her sponge baths.

“At my age, 89, I’m not a kid anymore and healing takes longer,” Feldman says.

“I don’t even feel like being with people. I just sit at home.”

Jasmine Jiles and three-year-old Max, Kahnawake Mohawk Territory, Que.

Jasmine Jiles says her three-year-old son Max came down with flu-like symptoms and cradled his ears in what she interpreted as a sign of pain, like the one pounding in her own head, around early July.

When Jiles heard about the recall soon after, she called Danone Canada, the plant-based milk manufacturer, to find out if their Silk coconut milk was in the contaminated batch. It was, she says.

“My son is very small, he’s very young, so I asked what we do in terms of overall monitoring and she said someone from the company would get in touch within 24 to 48 hours,” Jiles says from a First Nations reserve near Montreal.

“I never got a call back. I never got an email”

At home, her son’s fever broke after three days, but gas pains stuck with him, she says. It took a couple weeks for him to get back to normal.

“In hindsight, I should have taken him (to the hospital) but we just tried to see if we could nurse him at home because wait times are pretty extreme,” Jiles says, “and I don’t have child care at the moment.”

Joseph Desmond, 50, Sydney, N.S.

Joseph Desmond says he suffered a seizure and fell off his sofa on July 9. He went to the emergency room, where they ran an electroencephalogram (EEG) test, and then returned home. Within hours, he had a second seizure and went back to hospital.

His third seizure happened the next morning while walking to the nurse’s station.

In severe cases of listeriosis, bacteria can spread to the central nervous system and cause seizures, according to Health Canada.

“The last two months have really been a nightmare,” says Desmond, who has joined the proposed lawsuit.

When he returned home from the hospital, his daughter took a carton of Silk dark chocolate almond milk out of the fridge and asked if he had heard about the recall. By that point, Desmond says he was on his second two-litre carton after finishing the first in June.

“It was pretty scary. Terrifying. I honestly thought I was going to die.”

Cheryl McCombe, 63, Haliburton, Ont.

The morning after suffering a second episode of vomiting, feverish sweats and diarrhea in the middle of the night in early July, Cheryl McCombe scrolled through the news on her phone and came across the recall.

A few years earlier, McCombe says she started drinking plant-based milks because it seemed like a healthier choice to splash in her morning coffee. On June 30, she bought two cartons of Silk cashew almond milk.

“It was on the (recall) list. I thought, ‘Oh my God, I got listeria,’” McCombe says. She called her doctor’s office and visited an urgent care clinic hoping to get tested and confirm her suspicion, but she says, “I was basically shut down at the door.”

Public Health Ontario does not recommend listeria testing for infected individuals with mild symptoms unless they are at risk of developing severe illness, such as people who are immunocompromised, elderly, pregnant or newborn.

“No wonder they couldn’t connect the dots,” she adds, referencing that it took close to a year for public health officials to find the source of the outbreak.

“I am a woman in my 60s and sometimes these signs are of, you know, when you’re vomiting and things like that, it can be a sign in women of a bigger issue,” McCombe says. She was seeking confirmation that wasn’t the case.

Disappointed, with her stomach still feeling off, she says she decided to boost her gut health with probiotics. After a couple weeks she started to feel like herself.

But since then, McCombe says, “I’m back on Kawartha Dairy cream in my coffee.”

This report by The Canadian Press was first published Sept. 16, 2024.

Canadian Press health coverage receives support through a partnership with the Canadian Medical Association. CP is solely responsible for this content.

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B.C. mayors seek ‘immediate action’ from federal government on mental health crisis

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VANCOUVER – Mayors and other leaders from several British Columbia communities say the provincial and federal governments need to take “immediate action” to tackle mental health and public safety issues that have reached crisis levels.

Vancouver Mayor Ken Sim says it’s become “abundantly clear” that mental health and addiction issues and public safety have caused crises that are “gripping” Vancouver, and he and other politicians, First Nations leaders and law enforcement officials are pleading for federal and provincial help.

In a letter to Prime Minister Justin Trudeau and Premier David Eby, mayors say there are “three critical fronts” that require action including “mandatory care” for people with severe mental health and addiction issues.

The letter says senior governments also need to bring in “meaningful bail reform” for repeat offenders, and the federal government must improve policing at Metro Vancouver ports to stop illicit drugs from coming in and stolen vehicles from being exported.

Sim says the “current system” has failed British Columbians, and the number of people dealing with severe mental health and addiction issues due to lack of proper care has “reached a critical point.”

Vancouver Police Chief Adam Palmer says repeat violent offenders are too often released on bail due to a “revolving door of justice,” and a new approach is needed to deal with mentally ill people who “pose a serious and immediate danger to themselves and others.”

This report by The Canadian Press was first published Sept. 16, 2024

The Canadian Press. All rights reserved.

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