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Ancient DNA suggests woolly mammoths roamed the Earth more recently than previously thought – Phys.org

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Genetic material found in permafrost sediments from the Yukon contains rich information about ancient ecosystems. Credit: Julius Csotonyi/Government of Yukon

In 2010, small cores of permafrost sediments were collected by a team at the University of Alberta from gold mines in the Klondike region of central Yukon. They had remained in cold storage until paleogeneticists at the McMaster Ancient DNA Centre applied new genomics techniques to better understand the global extinction of megafauna that had culminated in North America some 12,700 years ago.

These tiny sediment samples contain an immense wealth of ancient environmental DNA from innumerable plants and animals that lived in those environments over millennia. These genetic microfossils originate from all components of an ecosystem—including bacteria, fungi, plants and animals—and serve as a time capsule of long-lost ecosystems, such as the mammoth-steppe, which disappeared around 13,000 years ago.

How exactly these ecosystems restructured so significantly, and why large animals seem to have been the most impacted by this shift has been an active area of scientific debate since the 18th century.

We can now use environmental DNA to help fill the gaps that have driven this debate.

Ancient DNA, cutting-edge technologies

Bacterial, fungal and unidentifiable DNA make up over 99.99 percent of an environmental sample. In our case, we wanted a way to selectively recover the much smaller fraction of ancient plant and animal DNA that would help us better understand the collapse of the mammoth-steppe ecosystem.

For my doctoral research, I was part of a team that developed a a new technique to extract, isolate, sequence and identify tiny fragments of ancient DNA from sediment.

Synthesis of dated bones, ancient environmental DNA and archaeological sites in Yukon and Alaska. Credit: Tyler J. Murchie

We analyzed these DNA fragments to track the shifting cast of plants and animals that lived in central Yukon over the past 30,000 years. We found evidence for the late survival of woolly mammoths and horses in the Klondike region, some 3,000 years later than expected.

We then expanded our analysis to include 21 previously collected permafrost cores from four sites in the Klondike region that date between 4,000 to 30,000 years ago.

With current technologies, we not only could identify which organisms a set of genetic microfossils came from. But we were also able to reassemble those fragments into genomes to study their evolutionary histories—solely from sediment.

Tremendous environmental change

The Pleistocene-Holocene transition, which occurred about 11,700 years ago, was a period of tremendous change across the globe. In eastern Beringia (the former Eurasian land bridge and unglaciated regions of Yukon and Alaska), this period saw the collapse of the mammoth-steppe biome and its gradual replacement with the boreal forest as we know it today.

This brought about the loss of iconic ice age megaherbivores like the woolly mammoth, Yukon horse, and steppe bison, along with predators such as the American scimitar cat and Beringian lion, among many others.

We found ancient environmental DNA from a diverse spectrum of ancient fauna, including woolly mammoths, horses, steppe bison, caribou, rodents, birds and many other animals.

An evolutionary tree showing the location and relationship of horses and their relatives with genomes reconstructed from bones and sediment. Credit: Tyler J.Murchie, Author provided

We were also able to observe how ecosystems shifted with the rise of woody shrubs around 13,500 years ago, and how that correlated with a decline of DNA from woolly mammoths, horses and steppe bison. With this remarkably rich dataset, we observed four main findings.

  1. There was a surprising consistency in the signal between sites, suggesting our data was representative of ecological trends in the region.
  2. Woolly mammoth DNA declines prior to the Bølling–Allerød warming, a warm period at the end of the last ice age, suggesting that megafaunal losses may have been staggered.
  3. Forbs (herbaceous flowering plants) make up a substantial component of the mammoth-steppe ecosystem alongside grasses.
  4. There is a consistent signal of and Yukon horse persistence into the Holocene, as much as 7,000 years after their disappearance from fossil records.

When paired with other records, our genetic reconstructions suggest that the transition out of the last glacial period may have been more drawn out than dated bones alone would suggest.

Mammoths, for example, may have declined in local population abundance thousands of years earlier than other megafauna, which is potentially correlated with the first controversial evidence of humans in the area. Further, grassland grazing animals may have persisted for thousands of years in refugia (habitats that support the existence of an isolated population), despite the environmental shift.

Woolly mammoths alongside humans

Our data suggest that horses and woolly mammoths may have persisted in the Klondike until approximately 9,000 years ago and perhaps as recently as 5,700 years ago, outliving their supposed disappearance from local fossil records by 7,000 years. However, it is possible for ancient environmental DNA to survive erosion and re-deposition, which could mix the genetic signals of different time periods, necessitating a degree of caution in our interpretations.

Until recently, there was no evidence of survival into the mid-Holocene. But studies have now shown that mammoths survived until 5,500 and 4,000 years ago on Arctic islands.

Researchers in Denmark found evidence of woolly rhinoceroses surviving in Siberia at least 9,800 years ago. Credit: Shutterstock

Researchers at the Centre for GeoGenetics in Copenhagen found evidence for the late survival of horses and mammoths in Alaska until as recently as as 7,900 years ago. They also found evidence of mammoths surviving as recently as 3,900 years ago in Siberia, alongside woolly rhinoceros to at least 9,800 years ago.

Steppe bison, which were thought to have disappeared and been replaced by the American bison during the Pleistocene, have likewise been found to have survived even as recently as perhaps just 400 years ago. We were able to observe the presence of distinct genetic lineages of both woolly mammoths and steppe bison in the same sediment samples, which suggests that there were likely distinct populations of these animals living in the same area.

There is a growing body of evidence that many ice age megafauna probably survived well into recorded human history, roaming the north during the Bronze Age and while builders worked on the pyramids of Egypt.

Genetic archives of our ecological past

The growing sophistication of environmental DNA methods to study ancient genetic microfossils highlights just how much information is buried in sediments.

Permafrost is ideal for preserving ancient DNA, but as this perennially frozen ground thaws and degrades with a warming Arctic, so too will the genetic material preserved within, and the evolutionary mysteries they once held.

Advances in paleogenetics continues to push the boundaries of what was once relegated to science fiction. Who knows what undiscovered evolutionary information remains frozen in ordinary sediments, hidden in microfossils of ancient DNA?


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Ancient DNA found in soil samples reveals mammoths, Yukon wild horses survived thousands of years longer than believed


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Here’s how Helene and other storms dumped a whopping 40 trillion gallons of rain on the South

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More than 40 trillion gallons of rain drenched the Southeast United States in the last week from Hurricane Helene and a run-of-the-mill rainstorm that sloshed in ahead of it — an unheard of amount of water that has stunned experts.

That’s enough to fill the Dallas Cowboys’ stadium 51,000 times, or Lake Tahoe just once. If it was concentrated just on the state of North Carolina that much water would be 3.5 feet deep (more than 1 meter). It’s enough to fill more than 60 million Olympic-size swimming pools.

“That’s an astronomical amount of precipitation,” said Ed Clark, head of the National Oceanic and Atmospheric Administration’s National Water Center in Tuscaloosa, Alabama. “I have not seen something in my 25 years of working at the weather service that is this geographically large of an extent and the sheer volume of water that fell from the sky.”

The flood damage from the rain is apocalyptic, meteorologists said. More than 100 people are dead, according to officials.

Private meteorologist Ryan Maue, a former NOAA chief scientist, calculated the amount of rain, using precipitation measurements made in 2.5-mile-by-2.5 mile grids as measured by satellites and ground observations. He came up with 40 trillion gallons through Sunday for the eastern United States, with 20 trillion gallons of that hitting just Georgia, Tennessee, the Carolinas and Florida from Hurricane Helene.

Clark did the calculations independently and said the 40 trillion gallon figure (151 trillion liters) is about right and, if anything, conservative. Maue said maybe 1 to 2 trillion more gallons of rain had fallen, much if it in Virginia, since his calculations.

Clark, who spends much of his work on issues of shrinking western water supplies, said to put the amount of rain in perspective, it’s more than twice the combined amount of water stored by two key Colorado River basin reservoirs: Lake Powell and Lake Mead.

Several meteorologists said this was a combination of two, maybe three storm systems. Before Helene struck, rain had fallen heavily for days because a low pressure system had “cut off” from the jet stream — which moves weather systems along west to east — and stalled over the Southeast. That funneled plenty of warm water from the Gulf of Mexico. And a storm that fell just short of named status parked along North Carolina’s Atlantic coast, dumping as much as 20 inches of rain, said North Carolina state climatologist Kathie Dello.

Then add Helene, one of the largest storms in the last couple decades and one that held plenty of rain because it was young and moved fast before it hit the Appalachians, said University of Albany hurricane expert Kristen Corbosiero.

“It was not just a perfect storm, but it was a combination of multiple storms that that led to the enormous amount of rain,” Maue said. “That collected at high elevation, we’re talking 3,000 to 6000 feet. And when you drop trillions of gallons on a mountain, that has to go down.”

The fact that these storms hit the mountains made everything worse, and not just because of runoff. The interaction between the mountains and the storm systems wrings more moisture out of the air, Clark, Maue and Corbosiero said.

North Carolina weather officials said their top measurement total was 31.33 inches in the tiny town of Busick. Mount Mitchell also got more than 2 feet of rainfall.

Before 2017’s Hurricane Harvey, “I said to our colleagues, you know, I never thought in my career that we would measure rainfall in feet,” Clark said. “And after Harvey, Florence, the more isolated events in eastern Kentucky, portions of South Dakota. We’re seeing events year in and year out where we are measuring rainfall in feet.”

Storms are getting wetter as the climate change s, said Corbosiero and Dello. A basic law of physics says the air holds nearly 4% more moisture for every degree Fahrenheit warmer (7% for every degree Celsius) and the world has warmed more than 2 degrees (1.2 degrees Celsius) since pre-industrial times.

Corbosiero said meteorologists are vigorously debating how much of Helene is due to worsening climate change and how much is random.

For Dello, the “fingerprints of climate change” were clear.

“We’ve seen tropical storm impacts in western North Carolina. But these storms are wetter and these storms are warmer. And there would have been a time when a tropical storm would have been heading toward North Carolina and would have caused some rain and some damage, but not apocalyptic destruction. ”

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‘Big Sam’: Paleontologists unearth giant skull of Pachyrhinosaurus in Alberta

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It’s a dinosaur that roamed Alberta’s badlands more than 70 million years ago, sporting a big, bumpy, bony head the size of a baby elephant.

On Wednesday, paleontologists near Grande Prairie pulled its 272-kilogram skull from the ground.

They call it “Big Sam.”

The adult Pachyrhinosaurus is the second plant-eating dinosaur to be unearthed from a dense bonebed belonging to a herd that died together on the edge of a valley that now sits 450 kilometres northwest of Edmonton.

It didn’t die alone.

“We have hundreds of juvenile bones in the bonebed, so we know that there are many babies and some adults among all of the big adults,” Emily Bamforth, a paleontologist with the nearby Philip J. Currie Dinosaur Museum, said in an interview on the way to the dig site.

She described the horned Pachyrhinosaurus as “the smaller, older cousin of the triceratops.”

“This species of dinosaur is endemic to the Grand Prairie area, so it’s found here and nowhere else in the world. They are … kind of about the size of an Indian elephant and a rhino,” she added.

The head alone, she said, is about the size of a baby elephant.

The discovery was a long time coming.

The bonebed was first discovered by a high school teacher out for a walk about 50 years ago. It took the teacher a decade to get anyone from southern Alberta to come to take a look.

“At the time, sort of in the ’70s and ’80s, paleontology in northern Alberta was virtually unknown,” said Bamforth.

When paleontogists eventually got to the site, Bamforth said, they learned “it’s actually one of the densest dinosaur bonebeds in North America.”

“It contains about 100 to 300 bones per square metre,” she said.

Paleontologists have been at the site sporadically ever since, combing through bones belonging to turtles, dinosaurs and lizards. Sixteen years ago, they discovered a large skull of an approximately 30-year-old Pachyrhinosaurus, which is now at the museum.

About a year ago, they found the second adult: Big Sam.

Bamforth said both dinosaurs are believed to have been the elders in the herd.

“Their distinguishing feature is that, instead of having a horn on their nose like a triceratops, they had this big, bony bump called a boss. And they have big, bony bumps over their eyes as well,” she said.

“It makes them look a little strange. It’s the one dinosaur that if you find it, it’s the only possible thing it can be.”

The genders of the two adults are unknown.

Bamforth said the extraction was difficult because Big Sam was intertwined in a cluster of about 300 other bones.

The skull was found upside down, “as if the animal was lying on its back,” but was well preserved, she said.

She said the excavation process involved putting plaster on the skull and wooden planks around if for stability. From there, it was lifted out — very carefully — with a crane, and was to be shipped on a trolley to the museum for study.

“I have extracted skulls in the past. This is probably the biggest one I’ve ever done though,” said Bamforth.

“It’s pretty exciting.”

This report by The Canadian Press was first published Sept. 25, 2024.

The Canadian Press. All rights reserved.

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The ancient jar smashed by a 4-year-old is back on display at an Israeli museum after repair

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TEL AVIV, Israel (AP) — A rare Bronze-Era jar accidentally smashed by a 4-year-old visiting a museum was back on display Wednesday after restoration experts were able to carefully piece the artifact back together.

Last month, a family from northern Israel was visiting the museum when their youngest son tipped over the jar, which smashed into pieces.

Alex Geller, the boy’s father, said his son — the youngest of three — is exceptionally curious, and that the moment he heard the crash, “please let that not be my child” was the first thought that raced through his head.

The jar has been on display at the Hecht Museum in Haifa for 35 years. It was one of the only containers of its size and from that period still complete when it was discovered.

The Bronze Age jar is one of many artifacts exhibited out in the open, part of the Hecht Museum’s vision of letting visitors explore history without glass barriers, said Inbal Rivlin, the director of the museum, which is associated with Haifa University in northern Israel.

It was likely used to hold wine or oil, and dates back to between 2200 and 1500 B.C.

Rivlin and the museum decided to turn the moment, which captured international attention, into a teaching moment, inviting the Geller family back for a special visit and hands-on activity to illustrate the restoration process.

Rivlin added that the incident provided a welcome distraction from the ongoing war in Gaza. “Well, he’s just a kid. So I think that somehow it touches the heart of the people in Israel and around the world,“ said Rivlin.

Roee Shafir, a restoration expert at the museum, said the repairs would be fairly simple, as the pieces were from a single, complete jar. Archaeologists often face the more daunting task of sifting through piles of shards from multiple objects and trying to piece them together.

Experts used 3D technology, hi-resolution videos, and special glue to painstakingly reconstruct the large jar.

Less than two weeks after it broke, the jar went back on display at the museum. The gluing process left small hairline cracks, and a few pieces are missing, but the jar’s impressive size remains.

The only noticeable difference in the exhibit was a new sign reading “please don’t touch.”

The Canadian Press. All rights reserved.

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