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How children are spoofing Covid-19 tests with soft drinks – BBC News

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Some children have found a devious method to get out of school – using cola to create false positive Covid tests. How does it work?

Children are always going to find cunning ways to bunk off school, and the latest trick is to fake a positive Covid-19 lateral flow test (LFT) using soft drinks. [Videos of the trick have been circulating on TikTok since December and a school in Liverpool, UK, recently wrote to parents to warn them about it.] So how are fruit juices, cola and devious kids fooling the tests, and is there a way to tell a fake positive result from a real one? I’ve tried to find out.

First, I thought it best to check the claims, so I cracked open bottles of cola and orange juice, then deposited a few drops directly onto LFTs. Sure enough, a few minutes later, two lines appeared on each test, supposedly indicating the presence of the virus that causes Covid-19.

It’s worth understanding how the tests work. If you open up an LFT device, you’ll find a strip of paper-like material, called nitrocellulose, and a small red pad, hidden under the plastic casing below the T-line. Absorbed on the red pad are antibodies that bind to the Covid-19 virus. They are also attached to gold nanoparticles (tiny particles of gold actually appear red), which allow us to see where the antibodies are on the device. When you do a test, you mix your sample with a liquid buffer solution, ensuring the sample stays at an optimum pH, before dripping it on the strip.

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The fluid wicks up the nitrocellulose strip and picks up the gold and antibodies. The latter also bind to the virus, if present. Further up the strip, next to the T (for test), are more antibodies that bind the virus. But these antibodies are not free to move – they are stuck to the nitrocellulose. As the red smear of gold-labelled antibodies pass this second set of antibodies, these also grab hold of the virus. The virus is then bound to both sets of antibodies – leaving everything, including the gold, immobilised on a line next to the T on the device, indicating a positive test.

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Gold antibodies that haven’t bound to the virus carry on up the strip where they meet a third set of antibodies, not designed to pick up Covid-19, stuck at the C (for control) line. These trap the remaining gold particles, without having to do so via the virus. This final line is used to indicate the test has worked.

The acidity of many soft drinks and fruit juices can lead to false positives in the Covid-19 lateral flow test but still be negative with a PCR test (Credit: Mark Lorch)

So, how can a soft drink cause the appearance of a red T line? One possibility is that the drinks contain something that the antibodies recognise and bind to, just as they do to the virus. But this is rather unlikely. The reason antibodies are used in tests like these is that they are incredibly fussy about what they bind to. There’s all sorts of stuff in the snot and saliva collected by the swabs you take from the nose and mouth, and the antibodies totally ignore this mess of protein, other viruses and remains of your breakfast. So they aren’t going to react to the ingredients of a soft drink.

A much more likely explanation is that something in the drinks is affecting the function of the antibodies. A range of fluids, from fruit juice to cola, have been used to fool the tests, but they all have one thing in common – they are highly acidic. The citric acid in orange juice, phosphoric acid in cola and malic acid in apple juice give these beverages a pH between 2.5 and 4. These are pretty harsh conditions for antibodies, which have evolved to work largely within the bloodstream, with its almost neutral pH of about 7.4.

Maintaining an ideal pH for the antibodies is key to the correct function of the test, and that’s the job of the liquid buffer solution that you mix your sample with, provided with the test. The critical role of the buffer is highlighted by the fact that if you mix cola with the buffer – as shown in this debunking of an Australian politician’s claim that mass testing is worthless – then the LFTs behave exactly as you’d expect: negative for Covid-19.

So without the buffer, the antibodies in the test are fully exposed to the acidic pH of the beverages. And this has a dramatic effect on their structure and function. Antibodies are proteins, which are comprised of amino acid building blocks, attached together to form long, linear chains. These chains fold up into very specific structures. Even a small change to the chains can dramatically impact a protein’s function. These structures are maintained by a network of many thousands of interactions between the various parts of the protein. For example, negatively charged parts of a protein will be attracted to positively charged areas.

Many schools in the UK have used regular lateral flow testing to check whether pupils might be carrying the Covid-19 virus (Credit: Oli Scarff/AFP/Getty Images)

Many schools in the UK have used regular lateral flow testing to check whether pupils might be carrying the Covid-19 virus (Credit: Oli Scarff/AFP/Getty Images)

But in acidic conditions, the protein becomes increasingly positively charged. As a result, many of the interactions that hold the protein together are disrupted, the delicate structure of the protein is affected and it no longer functions correctly. In this case, the antibodies’ sensitivity to the virus is lost.

Given this, you might expect that the acidic drinks would result in completely blank tests. But denatured proteins are sticky beasts. All of those perfectly evolved interactions that would normally hold the protein together are now orphaned and looking for something to bind to. A likely explanation is that the immobilised antibodies at the T-line stick directly to the gold particles as they pass by, producing the notorious cola-induced false positive result.

Is there then a way to spot a fake positive test? The antibodies (like most proteins) are capable of refolding and regaining their function when they are returned to more favourable conditions. So I tried washing a test that had been dripped with cola with buffer solution, and sure enough the immobilised antibodies at the T-line regained normal function and released the gold particles, revealing the true negative result on the test.

Children, I applaud your ingenuity, but now that I’ve found a way to uncover your trickery I suggest you use your cunning to devise a set of experiments and test my hypothesis. Then we can publish your results in a peer-reviewed journal.

* Mark Lorch is a professor of chemistry and science communication at the University of Hull, UK.

This article originally appeared on The Conversation, and is republished under a Creative Commons licence. 

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Marine plankton could act as alert in mass extinction event: UVic researcher – Langley Advance Times

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A University of Victoria micropaleontologist found that marine plankton may act as an early alert system before a mass extinction occurs.

With help from collaborators at the University of Bristol and Harvard, Andy Fraass’ newest paper in the Nature journal shows that after an analysis of fossil records showed that plankton community structures change before a mass extinction event.

“One of the major findings of the paper was how communities respond to climate events in the past depends on the previous climate,” Fraass said in a news release. “That means that we need to spend a lot more effort understanding recent communities, prior to industrialization. We need to work out what community structure looked like before human-caused climate change, and what has happened since, to do a better job at predicting what will happen in the future.”

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According to the release, the fossil record is the most complete and extensive archive of biological changes available to science and by applying advanced computational analyses to the archive, researchers were able to detail the global community structure of the oceans dating back millions of years.

A key finding of the study was that during the “early eocene climatic optimum,” a geological era with sustained high global temperatures equivalent to today’s worst case global warming scenarios, marine plankton communities moved to higher latitudes and only the most specialized plankton remained near the equator, suggesting that the tropical temperatures prevented higher amounts of biodiversity.

“Considering that three billion people live in the tropics, the lack of biodiversity at higher temperatures is not great news,” paper co-leader Adam Woodhouse said in the release.

Next, the team plans to apply similar research methods to other marine plankton groups.

Read More: Global study, UVic researcher analyze how mammals responded during pandemic

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Scientists Say They Have Found New Evidence Of An Unknown Planet… – 2oceansvibe News

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In the new work, scientists looked at a set of trans-Neptunian objects, or TNOs, which is the technical term for those objects that sit out at the edge of the solar system, beyond Neptune

The new work looked at those objects that have their movement made unstable because they interact with the orbit of Neptune. That instability meant they were harder to understand, so typically astronomers looking at a possible Planet Nine have avoided using them in their analysis.

Researchers instead looked towards those objects and tried to understand their movements. And, Dr Bogytin claimed, the best explanation is that they result from another, undiscovered planet.

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The team carried out a host of simulations to understand how those objects’ orbits were affected by a variety of things, including the giant planets around them such as Neptune, the “Galactic tide” that comes from the Milky Way, and passing stars.

The best explanation was from the model that included Planet 9, however, Dr Bogytin said. They noted that there were other explanations for the behaviour of those objects – including the suggestion that other planets once influenced their orbit, but have since been removed – but claim that the theory of Planet 9 remains the best explanation.

A better understanding of the existence or not of Planet 9 will come when the Vera C Rubin Observatory is turned on, the authors note. The observatory is currently being built in Chile, and when it is turned on it will be able to scan the sky to understand the behaviour of those distant objects.

Planet Nine is theorised to have a mass about 10 times that of Earth and orbit about 20 times farther from the Sun on average than Neptune. It may take between 10,000 and 20,000 Earth years to make one full orbit around the Sun.

You may be tempted to ask how an entire planet could ‘hide’ in our solar system when we have zooming capabilities such as the new iPhone 15 has, but consider this: If Earth was the size of a marble, the edge of our solar system would be 11 kilometres away. That’s a lot of space to hide a planet.

[source:independent]

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Dragonfly: NASA Just Confirmed The Most Exciting Space Mission Of Your Lifetime – Forbes

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NASA has confirmed that its exciting Dragonfly mission, which will fly a drone-like craft around Saturn’s largest moon, Titan, will cost $3.35 billion and launch in July 2028.

Titan is the only other world in the solar system other than Earth that has weather and liquid on the surface. It has an atmosphere, rain, lakes, oceans, shorelines, valleys, mountain ridges, mesas and dunes—and possibly the building blocks of life itself. It’s been described as both a utopia and as deranged because of its weird chemistry.

Set to reach Titan in 2034, the Dragonfly mission will last for two years once its lander arrives on the surface. During the mission, a rotorcraft will fly to a new location every Titan day (16 Earth days) to take samples of the giant moon’s prebiotic chemistry. Here’s what else it will do:

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  • Search for chemical biosignatures, past or present, from water-based life to that which might use liquid hydrocarbons.
  • Investigate the moon’s active methane cycle.
  • Explore the prebiotic chemistry in the atmosphere and on the surface.

Spectacular Mission

“Dragonfly is a spectacular science mission with broad community interest, and we are excited to take the next steps on this mission,” said Nicky Fox, associate administrator of the Science Mission Directorate at NASA Headquarters in Washington. “Exploring Titan will push the boundaries of what we can do with rotorcraft outside of Earth.”

It comes in the wake of the Mars Helicopter, nicknamed Ingenuity, which flew 72 times between April 2021 and its final flight in January 2023 despite only being expected to make up to five experimental test flights over 30 days. It just made its final downlink of data this week.

Dense Atmosphere

However, Titan is a completely different environment to Mars. Titan has a dense atmosphere on Titan, which will make buoyancy simple. Gravity on Titan is just 14% of the Earth’s. It sees just 1% of the sunlight received by Earth.

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The atmosphere is 98% nitrogen and 2% methane. Its seas and lakes are not water but liquid ethane and methane. The latter is gas in Titan’s atmosphere, but on its surface, it exists as a liquid in rain, snow, lakes, and ice on its surface.

COVID-Affected

Dragonfly was a victim of the pandemic. Slated to cost $1 billion when it was selected in 2019, it was meant to launch in 2026 and arrive in 2034 after an eight-year cruise phase. However, after delays due to COVID, NASA decided to compensate for the inevitable delayed launch by funding a heavy-lift launch vehicle to massively shorten the mission’s cruise phase.

The end result is that Dragonfly will take off two years later but arrive on schedule.

Previous Visit

Dragonfly won’t be the first time a robotic probe has visited Titan. As part of NASA’s landmark Cassini mission to Saturn between 2004 and 2017, a small probe called Huygens was despatched into Titan’s clouds on January 14, 2005. The resulting timelapse movie of its 2.5 hours descent—which heralded humanity’s first-ever (and only) views of Titan’s surface—is a must-see for space fans. It landed in an area of rounded blocks of ice, but on the way down, it saw ancient dry shorelines reminiscent of Earth as well as rivers of methane.

The announcement by NASA makes July 2028 a month worth circling for space fans, with a long-duration total solar eclipse set for July 22, 2028, in Australia and New Zealand.

Wishing you clear skies and wide eyes.

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