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Paul Berg, pioneer in gene splicing who led way for biotech, dies at 96

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Paul Berg, a Nobel laureate biochemist whose breakthrough in splicing DNA molecules helped place the foundations for the biotech industry, but who was once so concerned about possible risks from manipulating genes that he asked scientists to allow government oversight, died Feb. 15 at his home on the Stanford University campus in California. He was 96.
Stanford announced the death in a statement. No cause was given.
Dr. Berg’s question – as he and other scientists in the 1950s and ’60s learned more about the double-helix structure of DNA – was whether it was possible to transfer, from one organism to another, bits of genetic information. Success would give biologists and medical researchers an entirely new tool kit, once considered only the realm of science fiction stories about cloning.

In 1972, he gave the answer. Dr. Berg published a paper in a scientific journal that revealed he had mixed DNA from E. coli bacteria and a virus, SV40, linked to tumors in monkeys and transmissible to humans. An uproar followed.

Medical ethicists questioned whether Dr. Berg was toying with the natural order by creating what became known as recombinant DNA. Public health officials and others wondered if swapping DNA could create new plagues or unleash environmental catastrophes. “Is this the answer to Dr. Frankenstein’s dream?” later asked Alfred Vellucci, the mayor of Cambridge, Mass., home of Harvard University and the Massachusetts Institute of Technology.

Dr. Berg, too, had worries. He paused his experiments with SV40 and E. coli, uneasy over intersplicing the DNA of a disease-causing virus and a common intestinal bacteria.

A 1974 letter Dr. Berg signed with 10 colleagues, published in the journal Science, noted “serious concern that some of these artificial recombinant DNA molecules could prove biologically hazardous.” The letter called for an international meeting of the scientific community to “deal with the potential biohazards of recombinant DNA molecules.”

The gathering took place in a former chapel in Pacific Grove, Calif., in February 1975 with more than 140 scientists from around the world. They agreed to a general set of principles that included limits on the types of genes used and safeguards to keep recombinant DNA confined to laboratories. The guidelines reached at the Asilomar Conference Center were adopted in 1976 by the National Institutes of Health and similar oversight groups in other countries.

Many of the ground rules set by the conference have been revised or dropped as researchers developed greater understanding of genetics. Yet in hindsight, the worst-case thinking of the early years was merited, many researchers say.

“We had to be terribly cautious,” George Rathmann, the former chief executive of the biotech firm Amgen, said in 2005. “You can’t put these things back in a bottle.”

Other participants, however, described Dr. Berg and others as overstating the possible risks from the gene-splicing discoveries.

“It was a reflection of the Vietnam era and earlier history,” Waclaw Szybalski, then a professor and geneticist at the University of Wisconsin at Madison, told Science News in 1985. “Physicists were guilty of the atomic bomb, and chemists were guilty of napalm. Biologists were trying very hard to be guilty of something.”

Dr. Berg stood by his caution at the time. “I couldn’t say there was zero risk,” he recalled several years after being awarded the Nobel Prize in chemistry in 1980. He shared the prize with two other genetic researchers, Walter Gilbert and Frederick Sanger.

The Nobel Committee noted how Dr. Berg’s pioneering experiment in transplanting DNA molecules “has resulted in the development of a new technology, often called genetic engineering or gene manipulation.”

That also brought major commercial opportunities for what became the biotech industry, ranging from genetically modified crops to hundreds of drugs and therapies. The early products in the 1980s included vaccines for types of hepatitis and insulin. Previously, insulin from animals such as cattle and pigs were used in human treatment.

Recombinant DNA has been used in monoclonal antibodies that can be used as part of covid treatment, and in the latest coronavirus vaccine, Novavax, which was given emergency approval by the U.S. Food and Drug Administration last year.

In gene therapy, researchers are exploring ways to use CRISPR-based technology – essentially genetic scissors that can insert, repair or edit genes – for conditions caused by genetic mutations such as cystic fibrosis, Duchenne muscular dystrophy and Huntington’s disease.

Dr. Berg did not patent his findings, allowing pharmaceutical companies and other researchers to advance his work.

“You did science,” he said, “because you loved it.”

– – –

Science club beginnings

Paul Berg was born June 30, 1926, in Brooklyn as one of three sons of a father who worked in clothing manufacturing and a mother who was a homemaker. In high school, his interest in research was first kindled by a woman named Sophie Wolfe, who ran the science club after classes, he recounted.

During World War II, he tried to enlist at 17 to become a Navy aviator but was turned down because of his age. He later did preliminary flight training while studying at Pennsylvania State University. He was called up during the war and served on ships in the Atlantic and Pacific. Dr. Berg graduated in 1948 from Penn State, and received his doctorate from Western Reserve University (now Case Western Reserve University) in 1952.

Dr. Berg did postdoctoral work in cancer research and was an assistant professor of microbiology at the Washington University School of Medicine from 1955 to 1959, when he accepted a position at Stanford’s medical school.

In the early 1980s he led a campaign that raised more than $50 million to build the Beckman Center for Molecular and Genetic Medicine, which opened in 1989. Dr. Berg served as director of the center until 2000.

In 2004, Dr. Berg was one of 20 Nobel laureates who signed an open letter asserting that the administration of President George W. Bush was blocking or distorting scientific evidence to support policy decisions. The letter cited omissions of climate change data or decisions to ignore scientific analysis that questioned White House claims over Iraq’s weapons capabilities before the U.S.-led invasion in 2003.

Dr. Berg married Mildred Levy in 1947; she died in 2021. Survivors include a son, John.

Dr. Berg gave another contribution to molecular biology: the lingo. A recurring joke in research circles refers to the moment of the gene-splicing discovery. Anything before that is “B.C.,” before cloning.

 

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Here’s how Helene and other storms dumped a whopping 40 trillion gallons of rain on the South

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More than 40 trillion gallons of rain drenched the Southeast United States in the last week from Hurricane Helene and a run-of-the-mill rainstorm that sloshed in ahead of it — an unheard of amount of water that has stunned experts.

That’s enough to fill the Dallas Cowboys’ stadium 51,000 times, or Lake Tahoe just once. If it was concentrated just on the state of North Carolina that much water would be 3.5 feet deep (more than 1 meter). It’s enough to fill more than 60 million Olympic-size swimming pools.

“That’s an astronomical amount of precipitation,” said Ed Clark, head of the National Oceanic and Atmospheric Administration’s National Water Center in Tuscaloosa, Alabama. “I have not seen something in my 25 years of working at the weather service that is this geographically large of an extent and the sheer volume of water that fell from the sky.”

The flood damage from the rain is apocalyptic, meteorologists said. More than 100 people are dead, according to officials.

Private meteorologist Ryan Maue, a former NOAA chief scientist, calculated the amount of rain, using precipitation measurements made in 2.5-mile-by-2.5 mile grids as measured by satellites and ground observations. He came up with 40 trillion gallons through Sunday for the eastern United States, with 20 trillion gallons of that hitting just Georgia, Tennessee, the Carolinas and Florida from Hurricane Helene.

Clark did the calculations independently and said the 40 trillion gallon figure (151 trillion liters) is about right and, if anything, conservative. Maue said maybe 1 to 2 trillion more gallons of rain had fallen, much if it in Virginia, since his calculations.

Clark, who spends much of his work on issues of shrinking western water supplies, said to put the amount of rain in perspective, it’s more than twice the combined amount of water stored by two key Colorado River basin reservoirs: Lake Powell and Lake Mead.

Several meteorologists said this was a combination of two, maybe three storm systems. Before Helene struck, rain had fallen heavily for days because a low pressure system had “cut off” from the jet stream — which moves weather systems along west to east — and stalled over the Southeast. That funneled plenty of warm water from the Gulf of Mexico. And a storm that fell just short of named status parked along North Carolina’s Atlantic coast, dumping as much as 20 inches of rain, said North Carolina state climatologist Kathie Dello.

Then add Helene, one of the largest storms in the last couple decades and one that held plenty of rain because it was young and moved fast before it hit the Appalachians, said University of Albany hurricane expert Kristen Corbosiero.

“It was not just a perfect storm, but it was a combination of multiple storms that that led to the enormous amount of rain,” Maue said. “That collected at high elevation, we’re talking 3,000 to 6000 feet. And when you drop trillions of gallons on a mountain, that has to go down.”

The fact that these storms hit the mountains made everything worse, and not just because of runoff. The interaction between the mountains and the storm systems wrings more moisture out of the air, Clark, Maue and Corbosiero said.

North Carolina weather officials said their top measurement total was 31.33 inches in the tiny town of Busick. Mount Mitchell also got more than 2 feet of rainfall.

Before 2017’s Hurricane Harvey, “I said to our colleagues, you know, I never thought in my career that we would measure rainfall in feet,” Clark said. “And after Harvey, Florence, the more isolated events in eastern Kentucky, portions of South Dakota. We’re seeing events year in and year out where we are measuring rainfall in feet.”

Storms are getting wetter as the climate change s, said Corbosiero and Dello. A basic law of physics says the air holds nearly 4% more moisture for every degree Fahrenheit warmer (7% for every degree Celsius) and the world has warmed more than 2 degrees (1.2 degrees Celsius) since pre-industrial times.

Corbosiero said meteorologists are vigorously debating how much of Helene is due to worsening climate change and how much is random.

For Dello, the “fingerprints of climate change” were clear.

“We’ve seen tropical storm impacts in western North Carolina. But these storms are wetter and these storms are warmer. And there would have been a time when a tropical storm would have been heading toward North Carolina and would have caused some rain and some damage, but not apocalyptic destruction. ”

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Follow AP’s climate coverage at https://apnews.com/hub/climate

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Follow Seth Borenstein on Twitter at @borenbears

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Associated Press climate and environmental coverage receives support from several private foundations. See more about AP’s climate initiative here. The AP is solely responsible for all content.

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‘Big Sam’: Paleontologists unearth giant skull of Pachyrhinosaurus in Alberta

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It’s a dinosaur that roamed Alberta’s badlands more than 70 million years ago, sporting a big, bumpy, bony head the size of a baby elephant.

On Wednesday, paleontologists near Grande Prairie pulled its 272-kilogram skull from the ground.

They call it “Big Sam.”

The adult Pachyrhinosaurus is the second plant-eating dinosaur to be unearthed from a dense bonebed belonging to a herd that died together on the edge of a valley that now sits 450 kilometres northwest of Edmonton.

It didn’t die alone.

“We have hundreds of juvenile bones in the bonebed, so we know that there are many babies and some adults among all of the big adults,” Emily Bamforth, a paleontologist with the nearby Philip J. Currie Dinosaur Museum, said in an interview on the way to the dig site.

She described the horned Pachyrhinosaurus as “the smaller, older cousin of the triceratops.”

“This species of dinosaur is endemic to the Grand Prairie area, so it’s found here and nowhere else in the world. They are … kind of about the size of an Indian elephant and a rhino,” she added.

The head alone, she said, is about the size of a baby elephant.

The discovery was a long time coming.

The bonebed was first discovered by a high school teacher out for a walk about 50 years ago. It took the teacher a decade to get anyone from southern Alberta to come to take a look.

“At the time, sort of in the ’70s and ’80s, paleontology in northern Alberta was virtually unknown,” said Bamforth.

When paleontogists eventually got to the site, Bamforth said, they learned “it’s actually one of the densest dinosaur bonebeds in North America.”

“It contains about 100 to 300 bones per square metre,” she said.

Paleontologists have been at the site sporadically ever since, combing through bones belonging to turtles, dinosaurs and lizards. Sixteen years ago, they discovered a large skull of an approximately 30-year-old Pachyrhinosaurus, which is now at the museum.

About a year ago, they found the second adult: Big Sam.

Bamforth said both dinosaurs are believed to have been the elders in the herd.

“Their distinguishing feature is that, instead of having a horn on their nose like a triceratops, they had this big, bony bump called a boss. And they have big, bony bumps over their eyes as well,” she said.

“It makes them look a little strange. It’s the one dinosaur that if you find it, it’s the only possible thing it can be.”

The genders of the two adults are unknown.

Bamforth said the extraction was difficult because Big Sam was intertwined in a cluster of about 300 other bones.

The skull was found upside down, “as if the animal was lying on its back,” but was well preserved, she said.

She said the excavation process involved putting plaster on the skull and wooden planks around if for stability. From there, it was lifted out — very carefully — with a crane, and was to be shipped on a trolley to the museum for study.

“I have extracted skulls in the past. This is probably the biggest one I’ve ever done though,” said Bamforth.

“It’s pretty exciting.”

This report by The Canadian Press was first published Sept. 25, 2024.

The Canadian Press. All rights reserved.

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The ancient jar smashed by a 4-year-old is back on display at an Israeli museum after repair

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TEL AVIV, Israel (AP) — A rare Bronze-Era jar accidentally smashed by a 4-year-old visiting a museum was back on display Wednesday after restoration experts were able to carefully piece the artifact back together.

Last month, a family from northern Israel was visiting the museum when their youngest son tipped over the jar, which smashed into pieces.

Alex Geller, the boy’s father, said his son — the youngest of three — is exceptionally curious, and that the moment he heard the crash, “please let that not be my child” was the first thought that raced through his head.

The jar has been on display at the Hecht Museum in Haifa for 35 years. It was one of the only containers of its size and from that period still complete when it was discovered.

The Bronze Age jar is one of many artifacts exhibited out in the open, part of the Hecht Museum’s vision of letting visitors explore history without glass barriers, said Inbal Rivlin, the director of the museum, which is associated with Haifa University in northern Israel.

It was likely used to hold wine or oil, and dates back to between 2200 and 1500 B.C.

Rivlin and the museum decided to turn the moment, which captured international attention, into a teaching moment, inviting the Geller family back for a special visit and hands-on activity to illustrate the restoration process.

Rivlin added that the incident provided a welcome distraction from the ongoing war in Gaza. “Well, he’s just a kid. So I think that somehow it touches the heart of the people in Israel and around the world,“ said Rivlin.

Roee Shafir, a restoration expert at the museum, said the repairs would be fairly simple, as the pieces were from a single, complete jar. Archaeologists often face the more daunting task of sifting through piles of shards from multiple objects and trying to piece them together.

Experts used 3D technology, hi-resolution videos, and special glue to painstakingly reconstruct the large jar.

Less than two weeks after it broke, the jar went back on display at the museum. The gluing process left small hairline cracks, and a few pieces are missing, but the jar’s impressive size remains.

The only noticeable difference in the exhibit was a new sign reading “please don’t touch.”

The Canadian Press. All rights reserved.

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