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The effects of hybrid immunity on humoral immune response following COVID-19 mRNA vaccination in a cohort of essential and frontline workers

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In a recent study published in Clinical Infectious Diseases, researchers longitudinally evaluated humoral immune responses to the second and third (booster) doses of coronavirus disease 2019 (COVID-19) mRNA (messenger ribonucleic acid) vaccines among individuals with and without prior history of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections.

Study: SARS-CoV-2 infection history and antibody response to three COVID-19 mRNA vaccine doses. Image Credit: chatuphot/Shutterstock

Background

Studies have documented the generation of adaptive immune responses to SARS-CoV-2 infections and vaccinations, boosted by third-dose administration, to protect against COVID-19 severity outcomes. However, data on the synergistic hybrid immune protection conferred by SARS-CoV-2 infection and COVID-19 vaccination, among individuals with and without prior SARS-CoV-2 exposure, booster dose timing, and the effect of hybrid immunity on the waning of humoral immunity are limited.

About the study

In the present prospective cohort study, researchers evaluated the impact of hybrid immune responses conferred by SARS-CoV-2 mRNA vaccines and prior COVID-19 history on humoral immunity.

The sample population comprised healthcare workers (76%), initial responders (five percent), and other frontline workers (20%) across six US states, i.e., Arizona, Florida, Minnesota, Oregon, Texas, and Utah. Serum samples were obtained from the participants at recruitment and after intervals of 11 weeks to 13 weeks. In addition, participants submitted serum samples within 21.0 days to 60.0 days after reverse transcription-polymerase chain reaction (RT-PCR)-confirmed COVID-19, and 14.0 days to 60.0 days post-every COVID-19 vaccination.

The decline of antibodies against SARS-CoV-2 Wuhan/Hu-1/2019 strain spike protein subunit 2 (S2) and RBD (receptor-binding domain) was evaluated till 9.0 months post-second vaccination using ELISA (enzyme-linked immunosorbent assay) and documented as AUC (area under the serial dilution curve) values.

The team compared pre- and post-booster dose serological antibody titers among individuals belonging to either of the three groups- (i) vaccination only (no infection, n=224); (ii) infection before vaccination (previous infection, n=123); and (iii) infection after the second dose and before booster dose (breakthrough infection, n=41).

In addition, individuals self-obtained middle turbinate nasal swabs every week and at the beginning of CLI (COVID-19-like illness) symptoms such as chills, fever, breathlessness, cough, diarrhea, sore throat, muscular ache, and altered taste or smell. The swabs were subjected to RT-RCR analysis to detect SARS-CoV-2. Furthermore, the participants filled out online surveys to provide data on illness duration, fever absence/presence, and medical consultations.

The team compared AUC value changes as fold changes by linear mixed modeling adjusted for homologous mRNA-1273 or BNT162b2 vaccinations. Prior COVID-19 history was recorded based on self-report, antigen test or RT-RCR test reports, or the reports of serological tests performed at study enrollment.

SARS-CoV-2 vaccination status was either self-documented or self-confirmed by vaccination cards. Additionally, areas in Oregon, Minnesota, Utah, and Texas reviewed electronic health records, state registries, and occupational health reports.

The team included 338 essential and frontline workers who were administered three mRNA vaccine doses in the pre-Omicron variant period between July 2020 and November 2021. Individuals infected with SARS-CoV-2 between the first and second vaccine doses or after two weeks of the second vaccination were excluded from the analysis.

Results

Most participants were women (69.0%), aged between 18.0 years and 39.0 years (58.0%). The interval between the second and third doses was eight months. Among the participants, 88% and 10% were homologous BNT162b2 vaccinees and mRNA-1273 vaccinees, respectively. Among vaccination-only individuals, after the second dose, geometric mean ratio (GMR) values comparing anti-S2 and anti-RBD titers were 1.8 and 2.6, respectively.

The corresponding titers were reduced by 32% and 55%, respectively, within 200 days after the second vaccine dose. After the booster dose, GMR values for the corresponding antibodies increased by 2.9-fold and 2.6-fold, respectively. Individuals infected within 3.0 months prior to booster vaccination showed no significant increase in antibody titers post-booster dose.

Among individuals with prior COVID-19 history, after the first dose, GMR values for anti-S2 and anti-RBD antibody titers in comparison to post-SARS-CoV-2 infection titers were 1.6 and 3.2, respectively. After the second dose, the corresponding GMR values compared to the first dose were 1.0 and 1.1, respectively.

Post-second vaccination, previously infected individuals showed consistently greater antibody titers over time than those vaccinated only till greater than or equal to 200.0 days after the second dose, significantly boosted by the third dose.

Of note, among recently infected individuals, the final antibody titers before the vaccine booster were comparable to antibody titers post-COVID-19 vaccination without any significant changes in anti-RBD or anti-S2 titers from pre- to post-booster vaccination.

Contrastingly, among individuals with breakthrough SARS-CoV-2 infections >90 days prior to the booster vaccination, anti-S2 and anti-RBD titers were 1.7-fold and 2.3-fold greater after the booster dose in comparison to the pre-booster dose end-titers.

Conclusion

Overall, the study findings showed that the booster dose of SARS-CoV-2 mRNA vaccines induced robust humoral immunological responses among prime-vaccinated individuals irrespective of whether the individuals had been infected >3.0 months earlier. However, there was no significant humoral immune boost by the third vaccine dose in individuals infected with SARS-CoV-2 <3.0 months before booster vaccination. The findings indicated a minimum 3.0-month interval post-SARS-CoV-2 infection and booster vaccination for maximal humoral benefit.

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What’s the greatest holiday gift: lips, hair, skin? Give the gift of great skin this holiday season

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Give the gift of great skin this holiday season

Skinstitut Holiday Gift Kits take the stress out of gifting

Toronto, October 31, 2024 – Beauty gifts are at the top of holiday wish lists this year, and Laser Clinics Canada, a leader in advanced beauty treatments and skincare, is taking the pressure out of seasonal shopping. Today, Laser Clincs Canada announces the arrival of its 2024 Holiday Gift Kits, courtesy of Skinstitut, the exclusive skincare line of Laser Clinics Group.

In time for the busy shopping season, the limited-edition Holiday Gifts Kits are available in Laser Clinics locations in the GTA and Ottawa. Clinics are conveniently located in popular shopping centers, including Hillcrest Mall, Square One, CF Sherway Gardens, Scarborough Town Centre, Rideau Centre, Union Station and CF Markville. These limited-edition Kits are available on a first come, first served basis.

“These kits combine our best-selling products, bundled to address the most relevant skin concerns we’re seeing among our clients,” says Christina Ho, Senior Brand & LAM Manager at Laser Clinics Canada. “With several price points available, the kits offer excellent value and suit a variety of gift-giving needs, from those new to cosmeceuticals to those looking to level up their skincare routine. What’s more, these kits are priced with a savings of up to 33 per cent so gift givers can save during the holiday season.

There are two kits to select from, each designed to address key skin concerns and each with a unique theme — Brightening Basics and Hydration Heroes.

Brightening Basics is a mix of everyday essentials for glowing skin for all skin types. The bundle comes in a sleek pink, reusable case and includes three full-sized products: 200ml gentle cleanser, 50ml Moisture Defence (normal skin) and 30ml1% Hyaluronic Complex Serum. The Brightening Basics kit is available at $129, a saving of 33 per cent.

Hydration Heroes is a mix of hydration essentials and active heroes that cater to a wide variety of clients. A perfect stocking stuffer, this bundle includes four deluxe products: Moisture 15 15 ml Defence for normal skin, 10 ml 1% Hyaluronic Complex Serum, 10 ml Retinol Serum and 50 ml Expert Squalane Cleansing Oil. The kit retails at $59.

In addition to the 2024 Holiday Gifts Kits, gift givers can easily add a Laser Clinic Canada gift card to the mix. Offering flexibility, recipients can choose from a wide range of treatments offered by Laser Clinics Canada, or they can expand their collection of exclusive Skinstitut products.

 

Brightening Basics 2024 Holiday Gift Kit by Skinstitut, available exclusively at Laser Clincs Canada clinics and online at skinstitut.ca.

Hydration Heroes 2024 Holiday Gift Kit by Skinstitut – available exclusively at Laser Clincs Canada clinics and online at skinstitut.ca.

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Here is how to prepare your online accounts for when you die

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LONDON (AP) — Most people have accumulated a pile of data — selfies, emails, videos and more — on their social media and digital accounts over their lifetimes. What happens to it when we die?

It’s wise to draft a will spelling out who inherits your physical assets after you’re gone, but don’t forget to take care of your digital estate too. Friends and family might treasure files and posts you’ve left behind, but they could get lost in digital purgatory after you pass away unless you take some simple steps.

Here’s how you can prepare your digital life for your survivors:

Apple

The iPhone maker lets you nominate a “ legacy contact ” who can access your Apple account’s data after you die. The company says it’s a secure way to give trusted people access to photos, files and messages. To set it up you’ll need an Apple device with a fairly recent operating system — iPhones and iPads need iOS or iPadOS 15.2 and MacBooks needs macOS Monterey 12.1.

For iPhones, go to settings, tap Sign-in & Security and then Legacy Contact. You can name one or more people, and they don’t need an Apple ID or device.

You’ll have to share an access key with your contact. It can be a digital version sent electronically, or you can print a copy or save it as a screenshot or PDF.

Take note that there are some types of files you won’t be able to pass on — including digital rights-protected music, movies and passwords stored in Apple’s password manager. Legacy contacts can only access a deceased user’s account for three years before Apple deletes the account.

Google

Google takes a different approach with its Inactive Account Manager, which allows you to share your data with someone if it notices that you’ve stopped using your account.

When setting it up, you need to decide how long Google should wait — from three to 18 months — before considering your account inactive. Once that time is up, Google can notify up to 10 people.

You can write a message informing them you’ve stopped using the account, and, optionally, include a link to download your data. You can choose what types of data they can access — including emails, photos, calendar entries and YouTube videos.

There’s also an option to automatically delete your account after three months of inactivity, so your contacts will have to download any data before that deadline.

Facebook and Instagram

Some social media platforms can preserve accounts for people who have died so that friends and family can honor their memories.

When users of Facebook or Instagram die, parent company Meta says it can memorialize the account if it gets a “valid request” from a friend or family member. Requests can be submitted through an online form.

The social media company strongly recommends Facebook users add a legacy contact to look after their memorial accounts. Legacy contacts can do things like respond to new friend requests and update pinned posts, but they can’t read private messages or remove or alter previous posts. You can only choose one person, who also has to have a Facebook account.

You can also ask Facebook or Instagram to delete a deceased user’s account if you’re a close family member or an executor. You’ll need to send in documents like a death certificate.

TikTok

The video-sharing platform says that if a user has died, people can submit a request to memorialize the account through the settings menu. Go to the Report a Problem section, then Account and profile, then Manage account, where you can report a deceased user.

Once an account has been memorialized, it will be labeled “Remembering.” No one will be able to log into the account, which prevents anyone from editing the profile or using the account to post new content or send messages.

X

It’s not possible to nominate a legacy contact on Elon Musk’s social media site. But family members or an authorized person can submit a request to deactivate a deceased user’s account.

Passwords

Besides the major online services, you’ll probably have dozens if not hundreds of other digital accounts that your survivors might need to access. You could just write all your login credentials down in a notebook and put it somewhere safe. But making a physical copy presents its own vulnerabilities. What if you lose track of it? What if someone finds it?

Instead, consider a password manager that has an emergency access feature. Password managers are digital vaults that you can use to store all your credentials. Some, like Keeper,Bitwarden and NordPass, allow users to nominate one or more trusted contacts who can access their keys in case of an emergency such as a death.

But there are a few catches: Those contacts also need to use the same password manager and you might have to pay for the service.

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Is there a tech challenge you need help figuring out? Write to us at onetechtip@ap.org with your questions.

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Pediatric group says doctors should regularly screen kids for reading difficulties

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The Canadian Paediatric Society says doctors should regularly screen children for reading difficulties and dyslexia, calling low literacy a “serious public health concern” that can increase the risk of other problems including anxiety, low self-esteem and behavioural issues, with lifelong consequences.

New guidance issued Wednesday says family doctors, nurses, pediatricians and other medical professionals who care for school-aged kids are in a unique position to help struggling readers access educational and specialty supports, noting that identifying problems early couldhelp kids sooner — when it’s more effective — as well as reveal other possible learning or developmental issues.

The 10 recommendations include regular screening for kids aged four to seven, especially if they belong to groups at higher risk of low literacy, including newcomers to Canada, racialized Canadians and Indigenous Peoples. The society says this can be done in a two-to-three-minute office-based assessment.

Other tips encourage doctors to look for conditions often seen among poor readers such as attention-deficit hyperactivity disorder; to advocate for early literacy training for pediatric and family medicine residents; to liaise with schools on behalf of families seeking help; and to push provincial and territorial education ministries to integrate evidence-based phonics instruction into curriculums, starting in kindergarten.

Dr. Scott McLeod, one of the authors and chair of the society’s mental health and developmental disabilities committee, said a key goal is to catch kids who may be falling through the cracks and to better connect families to resources, including quicker targeted help from schools.

“Collaboration in this area is so key because we need to move away from the silos of: everything educational must exist within the educational portfolio,” McLeod said in an interview from Calgary, where he is a developmental pediatrician at Alberta Children’s Hospital.

“Reading, yes, it’s education, but it’s also health because we know that literacy impacts health. So I think that a statement like this opens the window to say: Yes, parents can come to their health-care provider to get advice, get recommendations, hopefully start a collaboration with school teachers.”

McLeod noted that pediatricians already look for signs of low literacy in young children by way of a commonly used tool known as the Rourke Baby Record, which offers a checklist of key topics, such as nutrition and developmental benchmarks, to cover in a well-child appointment.

But he said questions about reading could be “a standing item” in checkups and he hoped the society’s statement to medical professionals who care for children “enhances their confidence in being a strong advocate for the child” while spurring partnerships with others involved in a child’s life such as teachers and psychologists.

The guidance said pediatricians also play a key role in detecting and monitoring conditions that often coexist with difficulty reading such as attention-deficit hyperactivity disorder, but McLeod noted that getting such specific diagnoses typically involves a referral to a specialist, during which time a child continues to struggle.

He also acknowledged that some schools can be slow to act without a specific diagnosis from a specialist, and even then a child may end up on a wait list for school interventions.

“Evidence-based reading instruction shouldn’t have to wait for some of that access to specialized assessments to occur,” he said.

“My hope is that (by) having an existing statement or document written by the Canadian Paediatric Society … we’re able to skip a few steps or have some of the early interventions present,” he said.

McLeod added that obtaining specific assessments from medical specialists is “definitely beneficial and advantageous” to know where a child is at, “but having that sort of clear, thorough assessment shouldn’t be a barrier to intervention starting.”

McLeod said the society was partly spurred to act by 2022’s “Right to Read Inquiry Report” from the Ontario Human Rights Commission, which made 157 recommendations to address inequities related to reading instruction in that province.

He called the new guidelines “a big reminder” to pediatric providers, family doctors, school teachers and psychologists of the importance of literacy.

“Early identification of reading difficulty can truly change the trajectory of a child’s life.”

This report by The Canadian Press was first published Oct. 23, 2024.

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