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Discovery Supports a Surprising New View of How Life on Earth Originated

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Newly described chemical reaction could have assembled DNA building blocks before life forms and their enzymes existed.

Discovery boosts theory that life on our planet arose from RNA-DNA mix.

Chemists at Scripps Research have made a discovery that supports a surprising new view of how life originated on our planet.

In a study published in the chemistry journal Angewandte Chemie, they demonstrated that a simple compound called diamidophosphate (DAP), which was plausibly present on Earth before life arose, could have chemically knitted together tiny DNA building blocks called deoxynucleosides into strands of primordial DNA.

The finding is the latest in a series of discoveries, over the past several years, pointing to the possibility that DNA and its close chemical cousin RNA arose together as products of similar chemical reactions, and that the first self-replicating molecules — the first life forms on Earth — were mixes of the two.

The discovery may also lead to new practical applications in chemistry and biology, but its main significance is that it addresses the age-old question of how life on Earth first arose. In particular, it paves the way for more extensive studies of how self-replicating DNA-RNA mixes could have evolved and spread on the primordial Earth and ultimately seeded the more mature biology of modern organisms.

“This finding is an important step toward the development of a detailed chemical model of how the first life forms originated on Earth,” says study senior author Ramanarayanan Krishnamurthy, PhD, associate professor of chemistry at Scripps Research.

The finding also nudges the field of origin-of-life chemistry away from the hypothesis that has dominated it in recent decades: The “RNA World” hypothesis posits that the first replicators were RNA-based, and that DNA arose only later as a product of RNA life forms.

Is RNA too sticky?

Krishnamurthy and others have doubted the RNA World hypothesis in part because RNA molecules may simply have been too “sticky” to serve as the first self-replicators.

A strand of RNA can attract other individual RNA building blocks, which stick to it to form a sort of mirror-image strand — each building block in the new strand binding to its complementary building block on the original, “template” strand. If the new strand can detach from the template strand, and, by the same process, start templating other new strands, then it has achieved the feat of self-replication that underlies life.

But while RNA strands may be good at templating complementary strands, they are not so good at separating from these strands. Modern organisms make enzymes that can force twinned strands of RNA — or DNA — to go their separate ways, thus enabling replication, but it is unclear how this could have been done in a world where enzymes didn’t yet exist.

A chimeric workaround

Krishnamurthy and colleagues have shown in recent studies that “chimeric” molecular strands that are part DNA and part RNA may have been able to get around this problem, because they can template complementary strands in a less-sticky way that permits them to separate relatively easily.

The chemists also have shown in widely cited papers in the past few years that the simple ribonucleoside and deoxynucleoside building blocks, of RNA and DNA respectively, could have arisen under very similar chemical conditions on the early Earth.

Moreover, in 2017 they reported that the organic compound DAP could have played the crucial role of modifying ribonucleosides and stringing them together into the first RNA strands. The new study shows that DAP under similar conditions could have done the same for DNA.

“We found, to our surprise, that using DAP to react with deoxynucleosides works better when the deoxynucleosides are not all the same but are instead mixes of different DNA ‘letters’ such as A and T, or G and C, like real DNA,” says first author Eddy Jiménez, PhD, a postdoctoral research associate in the Krishnamurthy lab.

“Now that we understand better how a primordial chemistry could have made the first RNAs and DNAs, we can start using it on mixes of ribonucleoside and deoxynucleoside building blocks to see what chimeric molecules are formed — and whether they can self-replicate and evolve,” Krishnamurthy says.

He notes that the work may also have broad practical applications. The artificial synthesis of DNA and RNA — for example in the “PCR” technique that underlies COVID-19 tests — amounts to a vast global business, but depends on enzymes that are relatively fragile and thus have many limitations. Robust, enzyme-free chemical methods for making DNA and RNA may end up being more attractive in many contexts, Krishnamurthy says.

Reference: “Prebiotic Phosphorylation and Concomitant Oligomerization of Deoxynucleosides to form DNA” by Eddy Jiménez, Clémentine Gibard and Ramanarayanan Krishnamurthy, 15 December 2020, Angewandte Chemie.
DOI: 10.1002/anie.202015910

Funding was provided by the Simons Foundation.

Source: – SciTechDaily

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New species of crested dinosaur identified in Mexico

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A team of palaeontologists in Mexico have identified a new species of dinosaur after finding its 72 million-year-old fossilized remains almost a decade ago, Mexico’s National Institute of Anthropology and History (INAH) said on Thursday.

The new species, named Tlatolophus galorum, was identified as a crested dinosaur after 80% of its skull was recovered, allowing experts to compare it to other dinosaurs of that type, INAH said.

The investigation, which also included specialists from the National Autonomous University of Mexico, began in 2013 with the discovery of an articulated tail in the north-central Mexican state of Coahuila, where other discoveries have been made.

“Once we recovered the tail, we continued digging below where it was located. The surprise was that we began to find bones such as the femur, the scapula and other elements,” said Alejandro Ramírez, a scientist involved in the discovery.

Later, the scientists were able to collect, clean and analyze other bone fragments from the front part of the dinosaur’s body.

The palaeontologists had in their possession the crest of the dinosaur, which was 1.32 meters long, as well as other parts of the skull: lower and upper jaws, palate and even a part known as the neurocranium, where the brain was housed, INAH said.

The Mexican anthropology body also explained the meaning of the name – Tlatolophus galorum – for the new species of dinosaur.

Tlatolophus is a mixture of two words, putting together a term from the indigenous Mexican language of Nahuatl that means “word” with the Greek term meaning “crest”. Galorum refers to the people linked to the research, INAH said.

 

(Reporting by Abraham Gonzalez; Writing by Drazen Jorgic; Editing by Ana Nicolaci da Costa)

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Alberta family searches for answers in teen's sudden death after COVID exposure, negative tests – CBC.ca

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A southern Alberta mother and father are grappling with the sudden, unexplained death of their 17-year-old daughter, and with few answers, they’re left wondering if she could be the province’s youngest victim of COVID-19.

Sarah Strate — a healthy, active Grade 12 student at Magrath High School who loved singing, dancing and being outdoors — died on Monday, less than a week after being notified she’d been exposed to COVID-19.

While two tests came back negative, her parents say other signs point to the coronavirus, and they’re waiting for more answers. 

“It was so fast. It’s all still such a shock,” said Sarah’s mother, Kristine Strate. “She never even coughed. She had a sore throat and her ears were sore for a while, and [she had] swollen neck glands.”

Kristine said Sarah developed mild symptoms shortly after her older sister — who later tested positive for COVID-19 —  visited from Lethbridge, one of Alberta’s current hot spots for the virus.

The family went into isolation at their home in Magrath on Tuesday, April 20. They were swabbed the next day and the results were negative.

‘Everything went south, super-fast’

By Friday night, Sarah had developed fever and chills. On Saturday, she started vomiting and Kristine, a public health nurse, tried to keep her hydrated.

“She woke up feeling a bit more off on Monday morning,” Kristine said. “And everything went south, super-fast.”

Sarah had grown very weak and her parents decided to call 911 when she appeared to become delirious.

“She had her blanket on and I was talking to her and, in an instant, she was unresponsive,” said Kristine, who immediately started performing CPR on her daughter.

When paramedics arrived 20 minutes later, they were able to restore a heartbeat and rushed Sarah to hospital in Lethbridge, where she died.

“I thought there was hope once we got her heart rate back. I really did,” recalled Sarah’s father, Ron.

“He was praying for a miracle, and sometimes miracles don’t come,” said Kristine.

Strate’s parents say her health deteriorated quickly after being exposed to COVID-19. She died at Chinook Regional Hospital in Lethbridge on Monday. (Ron Strate)

Searching for answers

At the hospital, the family was told Sarah’s lungs were severely infected and that she may have ended up with blood clots in both her heart and lungs, a condition that can be a complication of COVID-19.

But a second test at the hospital came back negative for COVID-19.

“There really is no other answer,” Ron said. “When a healthy 17-year-old girl, who was sitting up in her bed and was able to talk, and within 10 minutes is unconscious on our floor — there was no reason [for it].”

The province currently has no record of any Albertans under the age of 20 who have died of COVID-19.

According to the Strate family, the medical examiner is running additional blood and tissue tests, in an effort to uncover the cause of Sarah’s death.

‘Unusual but not impossible’

University of Alberta infectious disease specialist Dr. Lynora Saxinger, who was not involved in Sarah’s treatment, says it is conceivable that further testing could uncover evidence of a COVID-19 infection, despite two negative test results.

However, she hasn’t seen a similar case in Alberta.

“It would be unusual but not impossible because no test is perfect. We have had cases where an initial test is negative and then if you keep on thinking it’s COVID and you re-test, you then can find COVID,” she said.

According to Saxinger, the rate of false negatives is believed to be very low. But it can happen if there are problems with the testing or specimen collection.

She says people are more likely to test positive after symptoms develop. 

“The best sensitivity of the test is around day four or five of having symptoms,” she said. “So you can miss things if you test very, very early. And with new development of symptoms, it’s always a good time to re-test because then the likelihood of getting a positive test is a little higher. But again, no test is perfect.” 

Sarah deteriorated so quickly — dying five days after she first developed symptoms — she didn’t live long enough to make it to her follow-up COVID-19 test. Instead, it was done at the hospital.

‘An amazing kid’

The Strate family now faces an agonizing wait for answers — one that will likely take months — about what caused Sarah’s death.

But Ron, who teaches at the school where Sarah attended Grade 12, wants his daughter to be remembered for the life she lived, not her death.

Strate, pictured here at three years old, had plans to become a massage therapist. She attended Grade 12 at Magrath High School and was an active, healthy teenager who was involved in sports, music and the school’s suicide prevention group. (Ron Strate)

Sarah was one of five children. Ron says she was strong, active and vibrant and had plans to become a massage therapist after graduating from high school.

She played several sports and loved to sing and dance as part of a show choir. She was a leader in the school’s suicide prevention group and would stand up for other students who were facing bullying.

“She’s one of the leaders in our Hope Squad … which goes out and helps kids to not be scared,” he father said.

“She’s an amazing kid.”

Sarah would often spend hours helping struggling classmates, and her parents hope her kindness is not forgotten.

“She’d done so many good things. Honestly, I’ve got so many messages from parents saying, ‘You have no idea how much your daughter helped our kid,'” said Ron.

“This 17-year-old girl probably lived more of a life in 17 years than most adults will live in their whole lives. She was so special. I love her so much.”

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China launches key module of space station planned for 2022

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BEIJING (Reuters) -China launched an unmanned module on Thursday containing what will become living quarters for three crew on a permanent space station that it plans to complete by the end of 2022, state media reported.

The module, named “Tianhe”, or “Harmony of the Heavens”, was launched on the Long March 5B, China’s largest carrier rocket, at 11:23 a.m. (0323 GMT) from the Wenchang Space Launch Centre on the southern island of Hainan.

Tianhe is one of three main components of what would be China’s first self-developed space station, rivalling the only other station in service – the International Space Station (ISS).

The ISS is backed by the United States, Russia, Europe, Japan and Canada. China was barred from participating by the United States.

“(Tianhe) is an important pilot project in the building of a powerful nation in both technology and in space,” state media quoted President Xi Jinping as saying in a congratulatory speech.

Tianhe forms the main living quarters for three crew members in the Chinese space station, which will have a life span of at least 10 years.

The Tianhe launch was the first of 11 missions needed to complete the space station, which will orbit Earth at an altitude of 340 to 450 km (211-280 miles).

In the later missions, China will launch the two other core modules, four manned spacecraft and four cargo spacecraft.

Work on the space station programme began a decade ago with the launch of a space lab Tiangong-1 in 2011, and later, Tiangong-2 in 2016.

Both helped China test the programme’s space rendezvous and docking capabilities.

China aims to become a major space power by 2030. It has ramped up its space programme with visits to the moon, the launch of an uncrewed probe to Mars and the construction of its own space station.

In contrast, the fate of the ageing ISS – in orbit for more than two decades – remains uncertain.

The project is set to expire in 2024, barring funding from its partners. Russia said this month that it would quit the project from 2025.

Russia is deepening ties with China in space as tensions with Washington rise.

Moscow has slammed the U.S.-led Artemis moon exploration programme and instead chosen to join Beijing in setting up a lunar research outpost in the coming years.

(Reporting by Ryan Woo and Liangping Gao; Editing by Christian Schmollinger, Simon Cameron-Moore and Lincoln Feast.)

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