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Growing eggs and sperm to treat infertility – News-Medical.net

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Scientists at the University of California, Los Angeles, have reported, in the journal Cell Reports, the mechanism of how the very early precursor or stem cells which eventually give rise to the germ cells, eggs and sperm, are formed over the course of development. This could help create eggs and sperms under laboratory conditions to help infertile people who don’t have sperms, or eggs, for instance.

UCLA Broad Stem Cell Research Center/Cell Reports – Differentiating human pluripotent stem cells (blue) turning into human germ cells (pink and white).

The problem

Infertility is a problem for 1 in 10 of the population in the US, and shows an increasing trend over the last few decades because of the later time of first pregnancy. In many of these cases, in vitro fertilization (IVF), and more advanced techniques like intracytoplasmic sperm injection (ICSI) are successfully used to begin a pregnancy. In IVF, the male and female germ cells are joined outside the body and the resulting conceptus is later inserted back into the uterus. With ICSI, the sperm nucleus is injected directly into the egg cell.

However, both of these techniques require sperm and eggs to be present. When it comes to people who lack these germ cells, other therapies must be looked for. The lack of sperm or eggs could be a result of genetic abnormalities, chemotherapy or radiation, or sometimes unknown causes.

One option is to use donor eggs or sperm, or both. However, as researcher Amander Clark says, “With donated eggs and sperm, the child is not genetically related to one or both parents. What we want to do is use stem cells to be able to generate germ cells outside the human body so that this kind of infertility can be overcome.”

The beginning

All embryos develop from a set of undifferentiated cells are present that is capable of developing in many different directions to make almost any kind of cell in the body. These are the pluripotent stem cells, the cells that can also give rise to sperms and eggs.

Scientists have already discovered how to make cells very similar to these from already differentiated adult skin or blood cells, by reversing the developmental timetable and un-differentiating them. These are called induced pluripotent stem cells (iPSCs).

The process

The current study made use of technology to measure the genes that were active in over 100,000 embryonic stem cells and iPSCs as they gave rise to sperm and egg cells. This huge amount of information was then analyzed, using newly developed algorithms from their colleagues at the Massachusetts Institute of Technology, to discern the pattern of development.

The scientists were thus able to come up with a high-definition picture of the process by which germ cells are formed. The first step occurred at 24-48 hours, when the stem cells begin to differentiate into the different types of cells that continue to develop into all the distinctive and highly specialized cells of the adult human body.

At the 12th day after fertilization, around the time of implantation but before the earliest formation of embryonic parts like the primitive streak or the gastrula, it is possible to recognize the earliest stem cells that are differentiating into the germ cell production line, called the human primordial germ cells (hPGCs). At this point the hPGC-like cells (hPGCLCs) are specified, or set apart, by a change in their gene expression towards a transitional state. At this point they share the characteristics of the pre-implantation stem cell as well as the post-implantation embryonic stem cell, which is described as naïve and primed, respectively. They then turn into the pathway to become germ cell progenitors, by the regulation of the sex-determining chromosome area SOX17 that is responsible for hPGCLC specification.

At this point, the hPGCLCs can no longer differentiate into somatic cells – this is called crossing Weismann’s barrier, after the legendary German biologist who proposed the existence of the hereditary factor within germ cells, today known to be DNA. This is the key point in achieving the in vitro production of gametes, or sex cells (eggs and sperms).

Implications

In the current experiment, the primed hPGCLCs are turned back to become transitional germinal pluripotent cells and thus begin to differentiate into germ cells. The researchers now know when to intervene so that they can maximize the number of germ cells formed by diverting more of the differentiation process into this stream.

Another interesting finding was that germ cells arise from stem cells originating in the amnion, the thin translucent membrane containing the fluid surrounding the embryo, as well as the gastrula-forming cells that belong to the baby proper.

Finally, they found that the gene activation patterns leading to germ cell formation are nearly the same, whether it is an embryonic stem cell or an iPSC. This proves they are using the right technique to form the germ cells.

Clark says, “Now we’re poised to take the next step of combining these cells with ovary or testis cells.” These germ cells are not yet decided on whether to develop into sperm or egg cells, and this depends on the molecular signals they receive, whether from the ovary or the testis.

The researchers say, “Through this work, we uncovered the human germline trajectory and discovered the identity of potential peri-implantation progenitors for hPGCs.”

The researchers hope they will eventually be able to coax the iPSCs formed from the patient’s own skin cells to differentiate into germ cells and into ovarian or testicular tissue. This could be used to let each person have his or her germ cells created in the laboratory. The process, however, is a long-drawn-out one and will require intensive research and work.

A step too far?

These techniques have been restricted to laboratory use, and are not approved by the US Food and Drug Administration for use in humans, nor have they undergone human testing.

It is obvious that such experiments involve the abundant use of human pre-implantation embryos. Though ethical approval norms are fulfilled, such testing nonetheless raises numerous other ethical issues. Again, the true motivation of the research remains in doubt since the lack of value for potential human life shown by such experiments makes it difficult to believe that making it possible for infertile couples to have babies is the real incentive.

Finally, the potential for misapplication of this technology is obvious, just one example being its use to allow both sperm and egg cells to be created from the same patient, nurtured by both ovarian and testicular tissue derived from that patient, and eventually giving rise to a single-parent zygote. One hopes that scientists will not allow their curiosity to run rampant but rein it in within appropriate bounds rather than ruin human life with unnecessary technology -simply because they can.

UCLA research could be step toward lab-grown eggs and sperm to treat infertility – http://newsroom.ucla.edu/releases/lab-grown-eggs-and-sperm-step-toward-infertility-treatment

Journal reference:

Human Primordial Germ Cells Are Specified from Lineage-Primed Progenitors Chen, Di et al. Cell Reports, Volume 29, Issue 13, 4568 – 4582.e5, https://www.cell.com/cell-reports/fulltext/S2211-1247(19)31574-8

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What’s the greatest holiday gift: lips, hair, skin? Give the gift of great skin this holiday season

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Give the gift of great skin this holiday season

Skinstitut Holiday Gift Kits take the stress out of gifting

Toronto, October 31, 2024 – Beauty gifts are at the top of holiday wish lists this year, and Laser Clinics Canada, a leader in advanced beauty treatments and skincare, is taking the pressure out of seasonal shopping. Today, Laser Clincs Canada announces the arrival of its 2024 Holiday Gift Kits, courtesy of Skinstitut, the exclusive skincare line of Laser Clinics Group.

In time for the busy shopping season, the limited-edition Holiday Gifts Kits are available in Laser Clinics locations in the GTA and Ottawa. Clinics are conveniently located in popular shopping centers, including Hillcrest Mall, Square One, CF Sherway Gardens, Scarborough Town Centre, Rideau Centre, Union Station and CF Markville. These limited-edition Kits are available on a first come, first served basis.

“These kits combine our best-selling products, bundled to address the most relevant skin concerns we’re seeing among our clients,” says Christina Ho, Senior Brand & LAM Manager at Laser Clinics Canada. “With several price points available, the kits offer excellent value and suit a variety of gift-giving needs, from those new to cosmeceuticals to those looking to level up their skincare routine. What’s more, these kits are priced with a savings of up to 33 per cent so gift givers can save during the holiday season.

There are two kits to select from, each designed to address key skin concerns and each with a unique theme — Brightening Basics and Hydration Heroes.

Brightening Basics is a mix of everyday essentials for glowing skin for all skin types. The bundle comes in a sleek pink, reusable case and includes three full-sized products: 200ml gentle cleanser, 50ml Moisture Defence (normal skin) and 30ml1% Hyaluronic Complex Serum. The Brightening Basics kit is available at $129, a saving of 33 per cent.

Hydration Heroes is a mix of hydration essentials and active heroes that cater to a wide variety of clients. A perfect stocking stuffer, this bundle includes four deluxe products: Moisture 15 15 ml Defence for normal skin, 10 ml 1% Hyaluronic Complex Serum, 10 ml Retinol Serum and 50 ml Expert Squalane Cleansing Oil. The kit retails at $59.

In addition to the 2024 Holiday Gifts Kits, gift givers can easily add a Laser Clinic Canada gift card to the mix. Offering flexibility, recipients can choose from a wide range of treatments offered by Laser Clinics Canada, or they can expand their collection of exclusive Skinstitut products.

 

Brightening Basics 2024 Holiday Gift Kit by Skinstitut, available exclusively at Laser Clincs Canada clinics and online at skinstitut.ca.

Hydration Heroes 2024 Holiday Gift Kit by Skinstitut – available exclusively at Laser Clincs Canada clinics and online at skinstitut.ca.

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Here is how to prepare your online accounts for when you die

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LONDON (AP) — Most people have accumulated a pile of data — selfies, emails, videos and more — on their social media and digital accounts over their lifetimes. What happens to it when we die?

It’s wise to draft a will spelling out who inherits your physical assets after you’re gone, but don’t forget to take care of your digital estate too. Friends and family might treasure files and posts you’ve left behind, but they could get lost in digital purgatory after you pass away unless you take some simple steps.

Here’s how you can prepare your digital life for your survivors:

Apple

The iPhone maker lets you nominate a “ legacy contact ” who can access your Apple account’s data after you die. The company says it’s a secure way to give trusted people access to photos, files and messages. To set it up you’ll need an Apple device with a fairly recent operating system — iPhones and iPads need iOS or iPadOS 15.2 and MacBooks needs macOS Monterey 12.1.

For iPhones, go to settings, tap Sign-in & Security and then Legacy Contact. You can name one or more people, and they don’t need an Apple ID or device.

You’ll have to share an access key with your contact. It can be a digital version sent electronically, or you can print a copy or save it as a screenshot or PDF.

Take note that there are some types of files you won’t be able to pass on — including digital rights-protected music, movies and passwords stored in Apple’s password manager. Legacy contacts can only access a deceased user’s account for three years before Apple deletes the account.

Google

Google takes a different approach with its Inactive Account Manager, which allows you to share your data with someone if it notices that you’ve stopped using your account.

When setting it up, you need to decide how long Google should wait — from three to 18 months — before considering your account inactive. Once that time is up, Google can notify up to 10 people.

You can write a message informing them you’ve stopped using the account, and, optionally, include a link to download your data. You can choose what types of data they can access — including emails, photos, calendar entries and YouTube videos.

There’s also an option to automatically delete your account after three months of inactivity, so your contacts will have to download any data before that deadline.

Facebook and Instagram

Some social media platforms can preserve accounts for people who have died so that friends and family can honor their memories.

When users of Facebook or Instagram die, parent company Meta says it can memorialize the account if it gets a “valid request” from a friend or family member. Requests can be submitted through an online form.

The social media company strongly recommends Facebook users add a legacy contact to look after their memorial accounts. Legacy contacts can do things like respond to new friend requests and update pinned posts, but they can’t read private messages or remove or alter previous posts. You can only choose one person, who also has to have a Facebook account.

You can also ask Facebook or Instagram to delete a deceased user’s account if you’re a close family member or an executor. You’ll need to send in documents like a death certificate.

TikTok

The video-sharing platform says that if a user has died, people can submit a request to memorialize the account through the settings menu. Go to the Report a Problem section, then Account and profile, then Manage account, where you can report a deceased user.

Once an account has been memorialized, it will be labeled “Remembering.” No one will be able to log into the account, which prevents anyone from editing the profile or using the account to post new content or send messages.

X

It’s not possible to nominate a legacy contact on Elon Musk’s social media site. But family members or an authorized person can submit a request to deactivate a deceased user’s account.

Passwords

Besides the major online services, you’ll probably have dozens if not hundreds of other digital accounts that your survivors might need to access. You could just write all your login credentials down in a notebook and put it somewhere safe. But making a physical copy presents its own vulnerabilities. What if you lose track of it? What if someone finds it?

Instead, consider a password manager that has an emergency access feature. Password managers are digital vaults that you can use to store all your credentials. Some, like Keeper,Bitwarden and NordPass, allow users to nominate one or more trusted contacts who can access their keys in case of an emergency such as a death.

But there are a few catches: Those contacts also need to use the same password manager and you might have to pay for the service.

___

Is there a tech challenge you need help figuring out? Write to us at onetechtip@ap.org with your questions.

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Pediatric group says doctors should regularly screen kids for reading difficulties

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The Canadian Paediatric Society says doctors should regularly screen children for reading difficulties and dyslexia, calling low literacy a “serious public health concern” that can increase the risk of other problems including anxiety, low self-esteem and behavioural issues, with lifelong consequences.

New guidance issued Wednesday says family doctors, nurses, pediatricians and other medical professionals who care for school-aged kids are in a unique position to help struggling readers access educational and specialty supports, noting that identifying problems early couldhelp kids sooner — when it’s more effective — as well as reveal other possible learning or developmental issues.

The 10 recommendations include regular screening for kids aged four to seven, especially if they belong to groups at higher risk of low literacy, including newcomers to Canada, racialized Canadians and Indigenous Peoples. The society says this can be done in a two-to-three-minute office-based assessment.

Other tips encourage doctors to look for conditions often seen among poor readers such as attention-deficit hyperactivity disorder; to advocate for early literacy training for pediatric and family medicine residents; to liaise with schools on behalf of families seeking help; and to push provincial and territorial education ministries to integrate evidence-based phonics instruction into curriculums, starting in kindergarten.

Dr. Scott McLeod, one of the authors and chair of the society’s mental health and developmental disabilities committee, said a key goal is to catch kids who may be falling through the cracks and to better connect families to resources, including quicker targeted help from schools.

“Collaboration in this area is so key because we need to move away from the silos of: everything educational must exist within the educational portfolio,” McLeod said in an interview from Calgary, where he is a developmental pediatrician at Alberta Children’s Hospital.

“Reading, yes, it’s education, but it’s also health because we know that literacy impacts health. So I think that a statement like this opens the window to say: Yes, parents can come to their health-care provider to get advice, get recommendations, hopefully start a collaboration with school teachers.”

McLeod noted that pediatricians already look for signs of low literacy in young children by way of a commonly used tool known as the Rourke Baby Record, which offers a checklist of key topics, such as nutrition and developmental benchmarks, to cover in a well-child appointment.

But he said questions about reading could be “a standing item” in checkups and he hoped the society’s statement to medical professionals who care for children “enhances their confidence in being a strong advocate for the child” while spurring partnerships with others involved in a child’s life such as teachers and psychologists.

The guidance said pediatricians also play a key role in detecting and monitoring conditions that often coexist with difficulty reading such as attention-deficit hyperactivity disorder, but McLeod noted that getting such specific diagnoses typically involves a referral to a specialist, during which time a child continues to struggle.

He also acknowledged that some schools can be slow to act without a specific diagnosis from a specialist, and even then a child may end up on a wait list for school interventions.

“Evidence-based reading instruction shouldn’t have to wait for some of that access to specialized assessments to occur,” he said.

“My hope is that (by) having an existing statement or document written by the Canadian Paediatric Society … we’re able to skip a few steps or have some of the early interventions present,” he said.

McLeod added that obtaining specific assessments from medical specialists is “definitely beneficial and advantageous” to know where a child is at, “but having that sort of clear, thorough assessment shouldn’t be a barrier to intervention starting.”

McLeod said the society was partly spurred to act by 2022’s “Right to Read Inquiry Report” from the Ontario Human Rights Commission, which made 157 recommendations to address inequities related to reading instruction in that province.

He called the new guidelines “a big reminder” to pediatric providers, family doctors, school teachers and psychologists of the importance of literacy.

“Early identification of reading difficulty can truly change the trajectory of a child’s life.”

This report by The Canadian Press was first published Oct. 23, 2024.

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