A group of researchers including a Concordia Ph.D. student have developed a new method of bioprinting adult neuron cells. They’re using a new laser-assisted technology that maintains high levels of cell viability and functionality.
Ph.D. candidate and 2020-21 Public Scholar Hamid Orimi and his co-authors present the feasibility of a new bioprinting technology they developed in a recent paper published in the journal Micromachines. They demonstrate how the methodology they created, called Laser-Induced Side Transfer (LIST), improves on existing bioprinting techniques by using bioinks of differing viscosities, allowing for better 3D printing. Orimi, his Concordia co-supervisor Sivakumar Narayanswamy in the Gina Cody School of Engineering and Computer Science, CRHMR co-supervisor Christos Boutopoulos and co-authors at the Université de Montréal first presented the method in the Nature journal Scientific Reports in 2020.
Orimi co-wrote the newer paper with lead author Katiane Roversi, Sebastien Talbot and Boutopoulos at UdeM and Marcelo Falchetti and Edroaldo da Rocha at Federal University of Santa Catarina in Brazil. In it, the researchers demonstrate that the technology can be used to successfully print sensory neurons, a vital component of the peripheral nervous system. This, they say, is promising for the long-term development of bioprinting’s potential, including disease modeling, drug testing and implant fabrication.
Viable and functional
The researchers used dorsal root ganglion (DRG) neurons from the peripheral nervous system of mice to test their technology. The neurons were suspended in a bioink solution and loaded into a square capillary above a biocompatible substrate. Low-energy nanosecond laser pulses were focused on the middle of the capillary, generating microbubbles that expanded and ejected a cell-laden microjet onto the substrate below it. The samples were briefly incubated, then washed and re-incubated for 48 hours.
The team then ran several tests to measure the printed cells’ capacities. A viability assay found that 86 percent of the cells remained alive two days after printing. The researchers note that viability rates improved when the laser used lower energy. The thermomechanics associated with higher laser energy use was more likely to damage the cells.
Other tests measured neurite outgrowth (in which developing neurons produce new projections as they grow in response to guidance cues), neuropeptide release, calcium imaging and RNA sequencing. Overall, the results were generally encouraging, suggesting that the technique could be an important contribution to the field of bioprinting.
Good for people and animals
“In general, people often leap to conclusions when we talk about bioprinting,” Orimi says. “They think that we can now print things like human organs for transplants. While this is a long-term objective, we are very far from that point. But there are still many ways to use this technology.”
Nearest at hand is drug discovery. The team hopes to get approval to continue their research into cell grafting, which can assist greatly in drug discovery, such as for nerve recovery medicines.
Another advantage to using this technology, Orimi says, is a decrease in animal testing. This not only has a humanitarian aspect—fewer animals will be euthanized to carry out experiments meant to benefit humans—but it will also produce more accurate results, since testing will be carried out on human, not animal, tissue.
Hamid Ebrahimi Orimi et al, Drop-on-demand cell bioprinting via Laser Induced Side Transfer (LIST), Scientific Reports (2020). DOI: 10.1038/s41598-020-66565-x
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Africa calls for climate finance tracker after donors fall short
African countries want a new system to track funding from wealthy nations that are failing to meet a $100-billion annual target to help the developing world tackle climate change, Africa’s lead climate negotiator said.
The demand highlights tensions ahead of the COP26 climate summit between the world’s 20 largest economies, which are behind 80% of greenhouse gas emissions, and developing countries that are bearing the brunt of the effects of global warming.
“If we prove that someone is responsible for something, it is his responsibility to pay for that,” said Tanguy Gahouma, chair of the African Group of Negotiators at COP26, the United Nations climate summit in Glasgow, Scotland, which starts on Oct. 31.
In 2009, developed countries agreed to raise $100 billion per year by 2020 to help the developing world deal with the fallout from a warming planet.
The latest available estimates from the Organisation for Economic Co-operation and Development (OECD) show this funding hit $79.6 billion in 2019, just 2% more than in 2018.
The OECD data shows Asian countries on average received 43% of the climate finance in 2016-19, while Africa received 26%. Gahouma said a more detailed shared system was needed that would keep tabs on each country’s contribution and where it went on the ground.
“They say they achieved maybe 70% of the target, but we cannot see that,” Gahouma said.
“We need to have a clear roadmap how they will put on the table the $100 billion per year, how we can track (it),” he said in an interview on Thursday. “We don’t have time to lose and Africa is one of the most vulnerable regions of the world.”
Temperatures in Africa are rising at a faster rate than the global average, according to the latest U.N. climate report. It forecasts further warming will lead to more extreme heatwaves, severe coastal flooding and intense rainfall on the continent.
Even as wealthy nations miss the $100 billion target, African countries plan to push for this funding to be scaled up more than tenfold by 2030.
“The $100 billion was a political commitment. It was not based on the real needs of developing countries to tackle climate change,” Gahouma said.
World leaders and their representatives have just a few days at the summit in Glasgow to try to broker deals to cut emissions faster and finance measures to adapt to climate pressures.
African countries face an extra challenge at the talks because administrative hurdles to entering Britain and to travelling during the coronavirus pandemic mean smaller than usual delegations can attend, Gahouma said.
“Limited delegations, with a very huge amount of work and limited time. This will be very challenging,” Gahouma said.
(Reporting by Alessandra Prentice; Editing by Aaron Ross and Janet Lawrence)
NASA Lucy Mission: Are Solar System Stem Cells Orbiting Jupiter? – The Press Stories
- Jonathan Amos
- Science Reporter
17 October 2021, 13:13 IST
A spacecraft was sent from Cape Canaveral to explore the fossil record in the solar system.
Lucy is a spacecraft orbiting Jupiter (Jupiter-Jupiter) to study clusters of two asteroids. One of them is in front of Jupiter in the orbit of the campus. The other is in the back.
NASA scientists say the study of these asteroids could help understand the effects of the first phase of solar system formation.
The Lucy spacecraft was launched from the Cape Canaveral in Florida at 9.45am on Saturday on an Atlas-5 rocket.
NASA initially decided to spend $ 98.1 billion (approximately Rs. 7,360 crore) on the mission over a twelve-year period.
There is a human fossil in Africa called Lucy. It was this fossil that helped us learn more about the existence of the human race.
Due to its inspiration, NASA carries out this mission under the same name.
“Trojan meteorites orbit Jupiter at 60 degrees,” said Hall Lewison of the Southwest Research Institute in Colorado. He was the leading researcher on the Lucy spacecraft.
“Under the influence of the gravitational pull of Jupiter and the Sun, these asteroids are constantly orbiting in that orbit. If any object falls there in the early days of the solar system, it will always be stable. So these fragments are fossils of what the planets are made of,” said Hall Louison.
Lucy explores many factors such as the shape, texture, surface conditions and composition of the materials that make up their pieces that are the size of the city or larger.
Jupiter is examining whether these fragments are derived from objects found on satellites.
“For example, if they were made of the same materials as we call the Khyber belt, we would assume that they are made of the Khyber belt and then rotate,” said Dr. Carly Howet, a Southwest Mission scientist. Research Institute.
Carly said the task was the result of some unusual navigation calculations.
Lucy will travel a total of 600 million kilometers. It will explore the Trojan complex in 2027/28. Jupiter will then reach the clusters of pieces on the other side in 2033.
When and where did Lucy go
The group ahead of Jupiter in orbit:
* Eurobates, Queta (Natural Satellite) – August 2027
* Polymel – September 2027
Lucas – April 2028
* Oras – November 2028
The group behind Jupiter in orbit:
* Petroclus, Menosius – March 2033
Key Belt Asteroids:
* Donald Johnson – April 2025
Astronauts capture stunning aurora from International Space Station – Space.com
Astronauts got to see an amazing display of southern lights over New Zealand and Antarctica earlier this month.
“I caught this aurora just as orbital sunrise was beginning. Breathtaking!” wrote NASA astronaut Shane Kimbrough on Oct. 12, two days after the show took place. With his tweet came a sweeping view of auroras over the barely lit limb of the Earth.
Auroras take place when charged particles from the sun, known as the solar wind, flow along the magnetic field lines of Earth and interact with our atmosphere. As the particles are deflected by the magnetic field to our planet’s poles, their interactions with the atmosphere dumps in energy and causes the atmosphere to glow.
Amazing auroras: Stunning northern lights photos
I caught this aurora just as orbital sunrise was beginning. Breathtaking! pic.twitter.com/8km6i4M5VjOctober 12, 2021
The sun is somewhat near the beginning of a solar cycle, which lasts about 11 years. Each cycle has a “maximum,” at which point there is more solar activity manifested as solar flares and coronal mass ejections, which can cause auroras if any particles flow in the right direction towards Earth.
While we’re not near that maximum phase right now, the astronauts had a great viewpoint from their orbit at approximately 250 miles (400 km) above Earth, with no interfering atmosphere in the way. That said, French astronaut Thomas Pesquet said eventually the sun stopped observations.
“The view in this #timelapse passes the #aurora to marvel at the stars and then be overwhelmed by a sunrise,” Pesquet wrote in a tweet posted on Sunday (Oct. 17).
Although the aurora is beautiful, it could accompany a real danger for astronauts: radiation. NASA has lifetime radiation protocols in place for its spaceflyers to protect against ill effects of radiation events in orbit, which can be associated with conditions such as cancer. The agency is also investigating the exposure for astronauts at future spaceflight destinations such as the moon and Mars.
💚🌊 Une aurore polaire, des étoiles et l’éblouissement final du lever de soleil : que demander de plus ? #BonneNuit.💚🌊 The view in this #timelapse passes the #aurora to marvel at the stars and then be overhwelmed by a sunrise. #MissionAlpha pic.twitter.com/M7LDGtqd5lOctober 17, 2021
Follow Elizabeth Howell on Twitter @howellspace. Follow us on Twitter @Spacedotcom and on Facebook.
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