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The planet is paying for your iced coffee but it doesn’t have to

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December 18, 2023 — 

Done with your iced coffee? Now what? Recycle, trash, or compost?

“Businesses in Manitoba have been switching to the compostable polylactic acid (PLA) bioplastic as a “green” alternative to single-use plastics. But the reality is not that simple”, says Jessica Marinelli and Camille Prefontaine. Marinelli [BScHons/20222] is a MSc student in Chemistry and Prefontaine is a 5th-year student in the BSc Honours Biology program in the Faculty of Science. They are part of the International Genetically Engineered Machine (iGEM) student group at the University of Manitoba. Their team recently presented Project PLAnet Zero at the iGEM 2023 Grand Jamboree in November 2023, won a silver medal, and was nominated for best part collection in the overgrad category.

1. What got you interested in improving PLA plastic breakdown in local composting systems?

As the Canadian government has started to phase out petroleum-based plastics to reduce carbon emissions and plastic waste in landfills, businesses in Manitoba have been switching to compostable polylactic acid (PLA) bioplastic as a “green” alternative. But the reality is not that simple. Degradation of PLA requires specific temperature and moisture conditions, challenging to maintain in our local composting facilities, especially during dry cold winters. In many cases, PLA does not break down effectively and is diverted to landfills.

After meeting with waste management experts, we saw this as the perfect opportunity to develop a bio-based solution that would help ensure “sustainable” plastic ends up where it belongs, in the compost!

2. Can you tell me about your solution?

At the core of our solution is the use of enzymes to digest PLA on the molecular level. Enzymes are biomolecules that help break down and build up materials.

To reduce the cost, we engineered a bacterial organism to make PLA-degrading enzymes and display them on the bacterial surface. Doing this will allow the enzymes to directly come into contact with PLA plastics and not be made inside bacteria cells which would require a lengthy and expensive separation process to purify them. Our whole-cell approach avoids this and enables cost-effective PLA plastic breakdown in local composting facilities.

To increase the efficiency and effectiveness of enzymatic degradation of PLA, we expanded nature’s toolset of amino acids. All living cells use the same standard 20 canonical amino acids to make proteins. Our team went beyond this set of 20 amino acids and used novel amino acids (non-canonical amino acids) to improve PLA-degrading enzymes. We use an amino acid called norleucine to help our enzymes bind better and break down materials faster and more efficiently. Our improved enzymes can allow for effective breakdown in the lower temperature conditions of food compost.

We worked on our solution alongside many experts and stakeholders in the waste management and composting community in Manitoba. Their feedback has been valuable and integral to our project. Our team is excited to continue building these partnerships to help us further develop PLAnet Zero in the next iGEM cycle.

3. Can you tell me about the award and what it means to you and your team to have won a silver medal and to have been nominated for best part collection in the overgrad category?

We are very proud of our Prairie iGEM team members for our silver medal win! We have all put so much work into this project over the past year and it was great to see our hard work pay off. To put this in context, our achievement is on par with leading Canadian institutes such as McMaster University and Queen’s University as well as top global institutes such as MIT and Harvard University. We have all put so much work into this project over the past year and it was great to see our hard work pay off.

This year, we were also nominated for best part collection (the development of a coherent group of parts that have a common function or theme) for our whole-cell catalyst design. The best part collection award is challenging because it requires a coherent vision and execution of the project. We are proud to be among the final 4 nominees out of 106 teams around the world in the overgrad category. To achieve this, we worked with a series of PLA-degrading enzymes and parts for our surface-display mechanism such that they could all be used collectively or interchangeably when designing a whole-cell catalyst for plastic degradation. Our results and success have shown that students at the University of Manitoba can conduct world-class research and have a lot to offer to the international Synthetic Biology community.

Every year we have seen more and more progress as the synthetic biology community on campus grows. PLAnet Zero allowed us to bring in students across all disciplines with a passion for science and sustainability and who want to implement real change in the community. It has been a really rewarding experience to see students experience science and entrepreneurship in the real world and see the future that synthetic biology has to offer to our local Winnipeg and global communities.

 

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Here’s how Helene and other storms dumped a whopping 40 trillion gallons of rain on the South

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More than 40 trillion gallons of rain drenched the Southeast United States in the last week from Hurricane Helene and a run-of-the-mill rainstorm that sloshed in ahead of it — an unheard of amount of water that has stunned experts.

That’s enough to fill the Dallas Cowboys’ stadium 51,000 times, or Lake Tahoe just once. If it was concentrated just on the state of North Carolina that much water would be 3.5 feet deep (more than 1 meter). It’s enough to fill more than 60 million Olympic-size swimming pools.

“That’s an astronomical amount of precipitation,” said Ed Clark, head of the National Oceanic and Atmospheric Administration’s National Water Center in Tuscaloosa, Alabama. “I have not seen something in my 25 years of working at the weather service that is this geographically large of an extent and the sheer volume of water that fell from the sky.”

The flood damage from the rain is apocalyptic, meteorologists said. More than 100 people are dead, according to officials.

Private meteorologist Ryan Maue, a former NOAA chief scientist, calculated the amount of rain, using precipitation measurements made in 2.5-mile-by-2.5 mile grids as measured by satellites and ground observations. He came up with 40 trillion gallons through Sunday for the eastern United States, with 20 trillion gallons of that hitting just Georgia, Tennessee, the Carolinas and Florida from Hurricane Helene.

Clark did the calculations independently and said the 40 trillion gallon figure (151 trillion liters) is about right and, if anything, conservative. Maue said maybe 1 to 2 trillion more gallons of rain had fallen, much if it in Virginia, since his calculations.

Clark, who spends much of his work on issues of shrinking western water supplies, said to put the amount of rain in perspective, it’s more than twice the combined amount of water stored by two key Colorado River basin reservoirs: Lake Powell and Lake Mead.

Several meteorologists said this was a combination of two, maybe three storm systems. Before Helene struck, rain had fallen heavily for days because a low pressure system had “cut off” from the jet stream — which moves weather systems along west to east — and stalled over the Southeast. That funneled plenty of warm water from the Gulf of Mexico. And a storm that fell just short of named status parked along North Carolina’s Atlantic coast, dumping as much as 20 inches of rain, said North Carolina state climatologist Kathie Dello.

Then add Helene, one of the largest storms in the last couple decades and one that held plenty of rain because it was young and moved fast before it hit the Appalachians, said University of Albany hurricane expert Kristen Corbosiero.

“It was not just a perfect storm, but it was a combination of multiple storms that that led to the enormous amount of rain,” Maue said. “That collected at high elevation, we’re talking 3,000 to 6000 feet. And when you drop trillions of gallons on a mountain, that has to go down.”

The fact that these storms hit the mountains made everything worse, and not just because of runoff. The interaction between the mountains and the storm systems wrings more moisture out of the air, Clark, Maue and Corbosiero said.

North Carolina weather officials said their top measurement total was 31.33 inches in the tiny town of Busick. Mount Mitchell also got more than 2 feet of rainfall.

Before 2017’s Hurricane Harvey, “I said to our colleagues, you know, I never thought in my career that we would measure rainfall in feet,” Clark said. “And after Harvey, Florence, the more isolated events in eastern Kentucky, portions of South Dakota. We’re seeing events year in and year out where we are measuring rainfall in feet.”

Storms are getting wetter as the climate change s, said Corbosiero and Dello. A basic law of physics says the air holds nearly 4% more moisture for every degree Fahrenheit warmer (7% for every degree Celsius) and the world has warmed more than 2 degrees (1.2 degrees Celsius) since pre-industrial times.

Corbosiero said meteorologists are vigorously debating how much of Helene is due to worsening climate change and how much is random.

For Dello, the “fingerprints of climate change” were clear.

“We’ve seen tropical storm impacts in western North Carolina. But these storms are wetter and these storms are warmer. And there would have been a time when a tropical storm would have been heading toward North Carolina and would have caused some rain and some damage, but not apocalyptic destruction. ”

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Follow AP’s climate coverage at https://apnews.com/hub/climate

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Follow Seth Borenstein on Twitter at @borenbears

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Associated Press climate and environmental coverage receives support from several private foundations. See more about AP’s climate initiative here. The AP is solely responsible for all content.

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‘Big Sam’: Paleontologists unearth giant skull of Pachyrhinosaurus in Alberta

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It’s a dinosaur that roamed Alberta’s badlands more than 70 million years ago, sporting a big, bumpy, bony head the size of a baby elephant.

On Wednesday, paleontologists near Grande Prairie pulled its 272-kilogram skull from the ground.

They call it “Big Sam.”

The adult Pachyrhinosaurus is the second plant-eating dinosaur to be unearthed from a dense bonebed belonging to a herd that died together on the edge of a valley that now sits 450 kilometres northwest of Edmonton.

It didn’t die alone.

“We have hundreds of juvenile bones in the bonebed, so we know that there are many babies and some adults among all of the big adults,” Emily Bamforth, a paleontologist with the nearby Philip J. Currie Dinosaur Museum, said in an interview on the way to the dig site.

She described the horned Pachyrhinosaurus as “the smaller, older cousin of the triceratops.”

“This species of dinosaur is endemic to the Grand Prairie area, so it’s found here and nowhere else in the world. They are … kind of about the size of an Indian elephant and a rhino,” she added.

The head alone, she said, is about the size of a baby elephant.

The discovery was a long time coming.

The bonebed was first discovered by a high school teacher out for a walk about 50 years ago. It took the teacher a decade to get anyone from southern Alberta to come to take a look.

“At the time, sort of in the ’70s and ’80s, paleontology in northern Alberta was virtually unknown,” said Bamforth.

When paleontogists eventually got to the site, Bamforth said, they learned “it’s actually one of the densest dinosaur bonebeds in North America.”

“It contains about 100 to 300 bones per square metre,” she said.

Paleontologists have been at the site sporadically ever since, combing through bones belonging to turtles, dinosaurs and lizards. Sixteen years ago, they discovered a large skull of an approximately 30-year-old Pachyrhinosaurus, which is now at the museum.

About a year ago, they found the second adult: Big Sam.

Bamforth said both dinosaurs are believed to have been the elders in the herd.

“Their distinguishing feature is that, instead of having a horn on their nose like a triceratops, they had this big, bony bump called a boss. And they have big, bony bumps over their eyes as well,” she said.

“It makes them look a little strange. It’s the one dinosaur that if you find it, it’s the only possible thing it can be.”

The genders of the two adults are unknown.

Bamforth said the extraction was difficult because Big Sam was intertwined in a cluster of about 300 other bones.

The skull was found upside down, “as if the animal was lying on its back,” but was well preserved, she said.

She said the excavation process involved putting plaster on the skull and wooden planks around if for stability. From there, it was lifted out — very carefully — with a crane, and was to be shipped on a trolley to the museum for study.

“I have extracted skulls in the past. This is probably the biggest one I’ve ever done though,” said Bamforth.

“It’s pretty exciting.”

This report by The Canadian Press was first published Sept. 25, 2024.

The Canadian Press. All rights reserved.

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The ancient jar smashed by a 4-year-old is back on display at an Israeli museum after repair

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TEL AVIV, Israel (AP) — A rare Bronze-Era jar accidentally smashed by a 4-year-old visiting a museum was back on display Wednesday after restoration experts were able to carefully piece the artifact back together.

Last month, a family from northern Israel was visiting the museum when their youngest son tipped over the jar, which smashed into pieces.

Alex Geller, the boy’s father, said his son — the youngest of three — is exceptionally curious, and that the moment he heard the crash, “please let that not be my child” was the first thought that raced through his head.

The jar has been on display at the Hecht Museum in Haifa for 35 years. It was one of the only containers of its size and from that period still complete when it was discovered.

The Bronze Age jar is one of many artifacts exhibited out in the open, part of the Hecht Museum’s vision of letting visitors explore history without glass barriers, said Inbal Rivlin, the director of the museum, which is associated with Haifa University in northern Israel.

It was likely used to hold wine or oil, and dates back to between 2200 and 1500 B.C.

Rivlin and the museum decided to turn the moment, which captured international attention, into a teaching moment, inviting the Geller family back for a special visit and hands-on activity to illustrate the restoration process.

Rivlin added that the incident provided a welcome distraction from the ongoing war in Gaza. “Well, he’s just a kid. So I think that somehow it touches the heart of the people in Israel and around the world,“ said Rivlin.

Roee Shafir, a restoration expert at the museum, said the repairs would be fairly simple, as the pieces were from a single, complete jar. Archaeologists often face the more daunting task of sifting through piles of shards from multiple objects and trying to piece them together.

Experts used 3D technology, hi-resolution videos, and special glue to painstakingly reconstruct the large jar.

Less than two weeks after it broke, the jar went back on display at the museum. The gluing process left small hairline cracks, and a few pieces are missing, but the jar’s impressive size remains.

The only noticeable difference in the exhibit was a new sign reading “please don’t touch.”

The Canadian Press. All rights reserved.

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