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Discovery of Life Deep beneath Sea May Inspire Search on Mars – Lab Manager Magazine

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Associate professor Yohey Suzuki at the University of Tokyo led the effort to develop a new way to prepare rock samples to search for life deep beneath the seafloor. This is an example of one of the thin slices of rock he prepared using special epoxy to ensure the rock held its shape while it was cut.

Caitlin Devor, University of Tokyo, CC BY 4.0

Newly discovered single-celled creatures living deep beneath the seafloor have given researchers clues about how they might find life on Mars. These bacteria were discovered living in tiny cracks inside volcanic rocks after researchers persisted over a decade of trial and error to find a new way to examine the rocks.

Researchers estimate that the rock cracks are home to a community of bacteria as dense as that of the human gut, about 10 billion bacterial cells per cubic centimeter (0.06 cubic inch). In contrast, the average density of bacteria living in mud sediment on the seafloor is estimated to be 100 cells per cubic centimeter.

“I am now almost over-expecting that I can find life on Mars. If not, it must be that life relies on some other process that Mars does not have, like plate tectonics,” said associate professor Yohey Suzuki from the University of Tokyo, referring to the movement of land masses around Earth most notable for causing earthquakes. Suzuki is first author of the research paper announcing the discovery, published in Communications Biology.

Magic of clay minerals

“I thought it was a dream, seeing such rich microbial life in rocks,” said Suzuki, recalling the first time he saw bacteria inside the undersea rock samples.

Undersea volcanoes spew out lava at approximately 1,200 degrees Celsius (2,200 degrees Fahrenheit), which eventually cracks as it cools down and becomes rock. The cracks are narrow, often less than 1 millimeter (0.04 inch) across. Over millions of years, those cracks fill up with clay minerals, the same clay used to make pottery. Somehow, bacteria find their way into those cracks and multiply.

“These cracks are a very friendly place for life. Clay minerals are like a magic material on Earth; if you can find clay minerals, you can almost always find microbes living in them,” explained Suzuki.

The microbes identified in the cracks are aerobic bacteria, meaning they use a process similar to how human cells make energy, relying on oxygen and organic nutrients.

“Honestly, it was a very unexpected discovery. I was very lucky, because I almost gave up,” said Suzuki.

Cruise for deep ocean samples

Yohey Suzuki from the University of Tokyo and collaborators from around Japan are the first to find life surviving in solid rocks deep beneath the seafloor.

Caitlin Devor, University of Tokyo, CC BY 4.0

Suzuki and his colleagues discovered the bacteria in rock samples that he helped collect in late 2010 during the Integrated Ocean Drilling Program (IODP). IODP Expedition 329 took a team of researchers from the tropical island of Tahiti in the middle of the Pacific Ocean to Auckland, New Zealand. The research ship anchored above three locations along the route across the South Pacific Gyre and used a metal tube 5.7 kilometers long to reach the ocean floor. Then, a drill cut down 125 meters below the seafloor and pulled out core samples, each about 6.2 centimeters across. The first 75 meters beneath the seafloor were mud sediment and then researchers collected another 40 meters of solid rock.

Depending on the location, the rock samples were estimated to be 13.5 million, 33.5 million and 104 million years old. The collection sites were not near any hydrothermal vents or sub-seafloor water channels, so researchers are confident the bacteria arrived in the cracks independently rather than being forced in by a current. The rock core samples were also sterilized to prevent surface contamination using an artificial seawater wash and a quick burn, a process Suzuki compares to making aburi (flame-seared) sushi.


Related Article: What Will a Lab on Mars Be Like?


At that time, the standard way to find bacteria in rock samples was to chip away the outer layer of the rock, then grind the center of the rock into a powder and count cells out of that crushed rock.

“I was making loud noises with my hammer and chisel, breaking open rocks while everyone else was working quietly with their mud,” he recalled.

How to slice a rock

Insert Image Here (remove this text once you’ve added an image)

Aerobic bacteria live densely packed into tunnels of clay minerals found in this sample of solid rock, collected from 122 meters beneath the seafloor. Image B is 1,000 times greater magnification than Image A. The left side photo in each image was taken using normal light and the right side photo was taken using fluorescent light. The solid basalt rock is gray, the clay minerals are orange, and the bacterial cells are green spheres.

Suzuki et al. 2020, DOI: 10.1038/s42003-020-0860-1, CC BY 4.0

Over the years, continuing to hope that bacteria might be present but unable to find any, Suzuki decided he needed a new way to look specifically at the cracks running through the rocks. He found inspiration in the way pathologists prepare ultrathin slices of body tissue samples to diagnose disease. Suzuki decided to coat the rocks in a special epoxy to support their natural shape so that they wouldn’t crumble when he sliced off thin layers.

These thin sheets of solid rock were then washed with dye that stains DNA and placed under a microscope.

The bacteria appeared as glowing green spheres tightly packed into tunnels that glow orange, surrounded by black rock. That orange glow comes from clay mineral deposits, the “magic material” giving bacteria an attractive place to live.

Whole genome DNA analysis identified the different species of bacteria that lived in the cracks. Samples from different locations had similar, but not identical, species of bacteria. Rocks at different locations are different ages, which may affect what minerals have had time to accumulate and therefore what bacteria are most common in the cracks.

Suzuki and his colleagues speculate that the clay mineral-filled cracks concentrate the nutrients that the bacteria use as fuel. This might explain why the density of bacteria in the rock cracks is eight orders of magnitude greater than the density of bacteria living freely in mud sediment where seawater dilutes the nutrients.

From the ocean floor to Mars

The clay minerals filling cracks in deep ocean rocks are likely similar to the minerals that may be in rocks now on the surface of Mars.

“Minerals are like a fingerprint for what conditions were present when the clay formed. Neutral to slightly alkaline levels, low temperature, moderate salinity, iron-rich environment, basalt rock—all of these conditions are shared between the deep ocean and the surface of Mars,” said Suzuki.

Suzuki’s research team is beginning a collaboration with NASA’s Johnson Space Center to design a plan to examine rocks collected from the Martian surface by rovers. Ideas include keeping the samples locked in a titanium tube and using a CT (computed tomography) scanner, a type of 3D X-ray, to look for life inside clay mineral-filled cracks.

“This discovery of life where no one expected it in solid rock below the seafloor may be changing the game for the search for life in space,” said Suzuki.

This press release was originally published on the University of Tokyo website

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Here’s how Helene and other storms dumped a whopping 40 trillion gallons of rain on the South

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More than 40 trillion gallons of rain drenched the Southeast United States in the last week from Hurricane Helene and a run-of-the-mill rainstorm that sloshed in ahead of it — an unheard of amount of water that has stunned experts.

That’s enough to fill the Dallas Cowboys’ stadium 51,000 times, or Lake Tahoe just once. If it was concentrated just on the state of North Carolina that much water would be 3.5 feet deep (more than 1 meter). It’s enough to fill more than 60 million Olympic-size swimming pools.

“That’s an astronomical amount of precipitation,” said Ed Clark, head of the National Oceanic and Atmospheric Administration’s National Water Center in Tuscaloosa, Alabama. “I have not seen something in my 25 years of working at the weather service that is this geographically large of an extent and the sheer volume of water that fell from the sky.”

The flood damage from the rain is apocalyptic, meteorologists said. More than 100 people are dead, according to officials.

Private meteorologist Ryan Maue, a former NOAA chief scientist, calculated the amount of rain, using precipitation measurements made in 2.5-mile-by-2.5 mile grids as measured by satellites and ground observations. He came up with 40 trillion gallons through Sunday for the eastern United States, with 20 trillion gallons of that hitting just Georgia, Tennessee, the Carolinas and Florida from Hurricane Helene.

Clark did the calculations independently and said the 40 trillion gallon figure (151 trillion liters) is about right and, if anything, conservative. Maue said maybe 1 to 2 trillion more gallons of rain had fallen, much if it in Virginia, since his calculations.

Clark, who spends much of his work on issues of shrinking western water supplies, said to put the amount of rain in perspective, it’s more than twice the combined amount of water stored by two key Colorado River basin reservoirs: Lake Powell and Lake Mead.

Several meteorologists said this was a combination of two, maybe three storm systems. Before Helene struck, rain had fallen heavily for days because a low pressure system had “cut off” from the jet stream — which moves weather systems along west to east — and stalled over the Southeast. That funneled plenty of warm water from the Gulf of Mexico. And a storm that fell just short of named status parked along North Carolina’s Atlantic coast, dumping as much as 20 inches of rain, said North Carolina state climatologist Kathie Dello.

Then add Helene, one of the largest storms in the last couple decades and one that held plenty of rain because it was young and moved fast before it hit the Appalachians, said University of Albany hurricane expert Kristen Corbosiero.

“It was not just a perfect storm, but it was a combination of multiple storms that that led to the enormous amount of rain,” Maue said. “That collected at high elevation, we’re talking 3,000 to 6000 feet. And when you drop trillions of gallons on a mountain, that has to go down.”

The fact that these storms hit the mountains made everything worse, and not just because of runoff. The interaction between the mountains and the storm systems wrings more moisture out of the air, Clark, Maue and Corbosiero said.

North Carolina weather officials said their top measurement total was 31.33 inches in the tiny town of Busick. Mount Mitchell also got more than 2 feet of rainfall.

Before 2017’s Hurricane Harvey, “I said to our colleagues, you know, I never thought in my career that we would measure rainfall in feet,” Clark said. “And after Harvey, Florence, the more isolated events in eastern Kentucky, portions of South Dakota. We’re seeing events year in and year out where we are measuring rainfall in feet.”

Storms are getting wetter as the climate change s, said Corbosiero and Dello. A basic law of physics says the air holds nearly 4% more moisture for every degree Fahrenheit warmer (7% for every degree Celsius) and the world has warmed more than 2 degrees (1.2 degrees Celsius) since pre-industrial times.

Corbosiero said meteorologists are vigorously debating how much of Helene is due to worsening climate change and how much is random.

For Dello, the “fingerprints of climate change” were clear.

“We’ve seen tropical storm impacts in western North Carolina. But these storms are wetter and these storms are warmer. And there would have been a time when a tropical storm would have been heading toward North Carolina and would have caused some rain and some damage, but not apocalyptic destruction. ”

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Follow AP’s climate coverage at https://apnews.com/hub/climate

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Follow Seth Borenstein on Twitter at @borenbears

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Associated Press climate and environmental coverage receives support from several private foundations. See more about AP’s climate initiative here. The AP is solely responsible for all content.

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‘Big Sam’: Paleontologists unearth giant skull of Pachyrhinosaurus in Alberta

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It’s a dinosaur that roamed Alberta’s badlands more than 70 million years ago, sporting a big, bumpy, bony head the size of a baby elephant.

On Wednesday, paleontologists near Grande Prairie pulled its 272-kilogram skull from the ground.

They call it “Big Sam.”

The adult Pachyrhinosaurus is the second plant-eating dinosaur to be unearthed from a dense bonebed belonging to a herd that died together on the edge of a valley that now sits 450 kilometres northwest of Edmonton.

It didn’t die alone.

“We have hundreds of juvenile bones in the bonebed, so we know that there are many babies and some adults among all of the big adults,” Emily Bamforth, a paleontologist with the nearby Philip J. Currie Dinosaur Museum, said in an interview on the way to the dig site.

She described the horned Pachyrhinosaurus as “the smaller, older cousin of the triceratops.”

“This species of dinosaur is endemic to the Grand Prairie area, so it’s found here and nowhere else in the world. They are … kind of about the size of an Indian elephant and a rhino,” she added.

The head alone, she said, is about the size of a baby elephant.

The discovery was a long time coming.

The bonebed was first discovered by a high school teacher out for a walk about 50 years ago. It took the teacher a decade to get anyone from southern Alberta to come to take a look.

“At the time, sort of in the ’70s and ’80s, paleontology in northern Alberta was virtually unknown,” said Bamforth.

When paleontogists eventually got to the site, Bamforth said, they learned “it’s actually one of the densest dinosaur bonebeds in North America.”

“It contains about 100 to 300 bones per square metre,” she said.

Paleontologists have been at the site sporadically ever since, combing through bones belonging to turtles, dinosaurs and lizards. Sixteen years ago, they discovered a large skull of an approximately 30-year-old Pachyrhinosaurus, which is now at the museum.

About a year ago, they found the second adult: Big Sam.

Bamforth said both dinosaurs are believed to have been the elders in the herd.

“Their distinguishing feature is that, instead of having a horn on their nose like a triceratops, they had this big, bony bump called a boss. And they have big, bony bumps over their eyes as well,” she said.

“It makes them look a little strange. It’s the one dinosaur that if you find it, it’s the only possible thing it can be.”

The genders of the two adults are unknown.

Bamforth said the extraction was difficult because Big Sam was intertwined in a cluster of about 300 other bones.

The skull was found upside down, “as if the animal was lying on its back,” but was well preserved, she said.

She said the excavation process involved putting plaster on the skull and wooden planks around if for stability. From there, it was lifted out — very carefully — with a crane, and was to be shipped on a trolley to the museum for study.

“I have extracted skulls in the past. This is probably the biggest one I’ve ever done though,” said Bamforth.

“It’s pretty exciting.”

This report by The Canadian Press was first published Sept. 25, 2024.

The Canadian Press. All rights reserved.

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The ancient jar smashed by a 4-year-old is back on display at an Israeli museum after repair

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TEL AVIV, Israel (AP) — A rare Bronze-Era jar accidentally smashed by a 4-year-old visiting a museum was back on display Wednesday after restoration experts were able to carefully piece the artifact back together.

Last month, a family from northern Israel was visiting the museum when their youngest son tipped over the jar, which smashed into pieces.

Alex Geller, the boy’s father, said his son — the youngest of three — is exceptionally curious, and that the moment he heard the crash, “please let that not be my child” was the first thought that raced through his head.

The jar has been on display at the Hecht Museum in Haifa for 35 years. It was one of the only containers of its size and from that period still complete when it was discovered.

The Bronze Age jar is one of many artifacts exhibited out in the open, part of the Hecht Museum’s vision of letting visitors explore history without glass barriers, said Inbal Rivlin, the director of the museum, which is associated with Haifa University in northern Israel.

It was likely used to hold wine or oil, and dates back to between 2200 and 1500 B.C.

Rivlin and the museum decided to turn the moment, which captured international attention, into a teaching moment, inviting the Geller family back for a special visit and hands-on activity to illustrate the restoration process.

Rivlin added that the incident provided a welcome distraction from the ongoing war in Gaza. “Well, he’s just a kid. So I think that somehow it touches the heart of the people in Israel and around the world,“ said Rivlin.

Roee Shafir, a restoration expert at the museum, said the repairs would be fairly simple, as the pieces were from a single, complete jar. Archaeologists often face the more daunting task of sifting through piles of shards from multiple objects and trying to piece them together.

Experts used 3D technology, hi-resolution videos, and special glue to painstakingly reconstruct the large jar.

Less than two weeks after it broke, the jar went back on display at the museum. The gluing process left small hairline cracks, and a few pieces are missing, but the jar’s impressive size remains.

The only noticeable difference in the exhibit was a new sign reading “please don’t touch.”

The Canadian Press. All rights reserved.

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