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Crumpled Graphene Makes Ultra-sensitive Cancer DNA Detector – Lab Manager Magazine

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Illinois researchers found that crumpling graphene in DNA sensors made it tens of thousands of times more sensitive, making it a feasible platform for liquid biopsy.

Image courtesy of Mohammad Heiranian

CHAMPAIGN, IL — Graphene-based biosensors could usher in an era of liquid biopsy, detecting DNA cancer markers circulating in a patient’s blood or serum. But current designs need a lot of DNA. In a new study, crumpling graphene makes it more than ten thousand times more sensitive to DNA by creating electrical “hot spots,” researchers at the University of Illinois at Urbana-Champaign found. 

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Crumpled graphene could be used in a wide array of biosensing applications for rapid diagnosis, the researchers said. They published their results in the journal Nature Communications.

“This sensor can detect ultra-low concentrations of molecules that are markers of disease, which is important for early diagnosis,” said study leader Rashid Bashir, a professor of bioengineering and the dean of the Grainger College of Engineering at Illinois. “It’s very sensitive, it’s low-cost, it’s easy to use, and it’s using graphene in a new way.”

While the idea of looking for telltale cancer sequences in nucleic acids, such as DNA or its cousin RNA, isn’t new, this is the first electronic sensor to detect very small amounts, such as might be found in a patient’s serum, without additional processing.

“When you have cancer, certain sequences are overexpressed. But rather than sequencing someone’s DNA, which takes a lot of time and money, we can detect those specific segments that are cancer biomarkers in DNA and RNA that are secreted from the tumors into the blood,” said Michael Hwang, the first author of the study and a postdoctoral researcher in the Holonyak Micro and Nanotechnology Lab at Illinois.

Graphene—a flat sheet of carbon one atom thick—is a popular, low-cost material for electronic sensors. However, nucleic-acid sensors developed so far require a process called amplification—isolating a DNA or RNA fragment and copying it many times in a test tube. This process is lengthy and can introduce errors. So Bashir’s group set out to increase graphene’s sensing power to the point of being able to test a sample without first amplifying the DNA.

Many other approaches to boosting graphene’s electronic properties have involved carefully crafted nanoscale structures. Rather than fabricate special structures, the Illinois group simply stretched out a thin sheet of plastic, laid the graphene on top of it, then released the tension in the plastic, causing the graphene to scrunch up and form a crumpled surface.

They tested the crumpled graphene’s ability to sense DNA and a cancer-related microRNA in both a buffer solution and in undiluted human serum, and saw the performance improve tens of thousands of times over flat graphene.


Related Article: Raman Spectroscopy: The Drive to Improve Cancer Screening and Diagnosis


“This is the highest sensitivity ever reported for electrical detection of a biomolecule. Before, we would need tens of thousands of molecules in a sample to detect it. With this device, we could detect a signal with only a few molecules,” Hwang said. “I expected to see some improvement in sensitivity, but not like this.”

To determine the reason for this boost in sensing power, mechanical science and engineering professor Narayana Aluru and his research group used detailed computer simulations to study the crumpled graphene’s electrical properties and how DNA physically interacted with the sensor’s surface.

They found that the cavities served as electrical hotspots, acting as a trap to attract and hold the DNA and RNA molecules.

“When you crumple graphene and create these concave regions, the DNA molecule fits into the curves and cavities on the surface, so more of the molecule interacts with the graphene and we can detect it,” said graduate student Mohammad Heiranian, a co-first author of the study. “But when you have a flat surface, other ions in the solution like the surface more than the DNA, so the DNA does not interact much with the graphene and we cannot detect it.”

In addition, crumpling the graphene created a strain in the material that changed its electrical properties, inducing a bandgap—an energy barrier that electrons must overcome to flow through the material—that made it more sensitive to the electrical charges on the DNA and RNA molecules.

“This bandgap potential shows that crumpled graphene could be used for other applications as well, such as nano circuits, diodes, or flexible electronics,” said Amir Taqieddin, a graduate student and coauthor of the paper.

Even though DNA was used in the first demonstration of crumpled graphene’s sensitivity for biological molecules, the new sensor could be tuned to detect a wide variety of target biomarkers. Bashir’s group is testing crumpled graphene in sensors for proteins and small molecules as well.

“Eventually the goal would be to build cartridges for a handheld device that would detect target molecules in a few drops of blood, for example, in the way that blood sugar is monitored,” Bashir said. “The vision is to have measurements quickly and in a portable format.”

This press release was originally published on the Illinois News Bureau website

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SpaceX launch marks 300th successful booster landing – Phys.org

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Credit: Unsplash/CC0 Public Domain

SpaceX sent up the 30th launch from the Space Coast for the year on the evening of April 23, a mission that also featured the company’s 300th successful booster recovery.

A Falcon 9 rocket carrying 23 of SpaceX’s Starlink internet satellites blasted off at 6:17 p.m. Eastern time from Cape Canaveral Space Force Station’s Space Launch Complex 40.

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The first-stage booster set a milestone of the 300th time a Falcon 9 or Falcon Heavy booster made a successful recovery landing, and the 270th time SpaceX has reflown a booster.

This particular booster made its ninth trip to space, a resume that includes one human spaceflight, Crew-6. It made its latest recovery landing downrange on the droneship Just Read the Instructions in the Atlantic Ocean.

The company’s first successful booster recovery came in December 2015, and it has not had a failed booster landing since February 2021.

The current record holder for flights flew 11 days ago making its 20th trip off the .

SpaceX has been responsible for all but two of the launches this year from either Kennedy Space Center or Cape Canaveral with United Launch Alliance having launched the other two.

SpaceX could knock out more launches before the end of the month, putting the Space Coast on pace to hit more than 90 by the end of the year, but the rate of launches by SpaceX is also set to pick up for the remainder of the year with some turnaround times at the Cape’s SLC-40 coming in less than three days.

That could amp up frequency so the Space Coast could surpass 100 launches before the end of the year, with the majority coming from SpaceX. It hosted 72 launches in 2023.

More launches from ULA are on tap as well, though, including the May 6 launch atop an Atlas V rocket of the Boeing CST-100 Starliner with a pair of NASA astronauts to the International Space Station.

ULA is also preparing for the second launch ever of its new Vulcan Centaur rocket, which recently received its second Blue Origin BE-4 engine and is just waiting on the payload, Sierra Space’s Dream Chaser spacecraft, to make its way to the Space Coast.

Blue Origin has its own it wants to launch this year as well, with New Glenn making its debut as early as September, according to SLD 45’s range manifest.

2024 Orlando Sentinel. Distributed by Tribune Content Agency, LLC.

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SpaceX launch marks 300th successful booster landing (2024, April 24)
retrieved 24 April 2024
from https://phys.org/news/2024-04-spacex-300th-successful-booster.html

This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no
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Wildlife Wednesday: loons are suffering as water clarity diminishes – Canadian Geographic

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The common loon, that icon of northern wilderness, is under threat from climate change due to declining water clarity. Published earlier this month in the journal Ecology, a study conducted by biologists from Chapman University and Rensselaer Polytechnic Institute in the U.S. has demonstrated the first clear evidence of an effect of climate change on this species whose distinct call is so tied to the soundscape of Canada’s lakes and wetlands.

Through the course of their research, the scientists found that July rainfall results in reduced July water clarify in loon territories in Northern Wisconsin. In turn, this makes it difficult for adult loons to find and capture their prey — mainly small fish — underwater, meaning they are unable to meet their chicks’ metabolic needs. Undernourished, the chicks face higher mortality rates. The consistent foraging techniques used by loons across their range means this impact is likely echoed wherever they are found — from Alaska to Canada to Iceland.

The researchers used Landsat imagery to find that there has been a 25-year consistent decline in water clarity, and during this period, body weights of adult loon and chicks alike have also declined. With July being the month of most rapid growth in young loons, the study also pinpointed water clarity in July as being the greatest predictor of loon body weight. 

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One explanation for why heavier rainfall leads to reduced water clarity is the rain might carry dissolved organic matter into lakes from adjacent streams and shoreline areas. Lawn fertilizers, pet waste and septic system leaks may also be to blame.

The researchers, led by Chapman University professor Walter Piper, hope to use these insights to further conservation efforts for this bird Piper describes as both “so beloved and so poorly understood.”

Return of the king

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Giant prehistoric salmon had tusk-like teeth for defence, building nests

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The artwork and publicity materials showcasing a giant salmon that lived five million years ago were ready to go to promote a new exhibit, when the discovery of two fossilized skulls immediately changed what researchers knew about the fish.

Initial fossil discoveries of the 2.7-metre-long salmon in Oregon in the 1970s were incomplete and had led researchers to mistakenly suggest the fish had fang-like teeth.

It was dubbed the “sabre-toothed salmon” and became a kind of mascot for the Museum of Natural and Cultural History at the University of Oregon, says researcher Edward Davis.

But then came discovery of two skulls in 2014.

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Davis, a member of the team that found the skulls, says it wasn’t until they got back to the lab that he realized the significance of the discovery that has led to the renaming of the fish in a new, peer-reviewed study.

“There were these two skulls staring at me with sideways teeth,” says Davis, an associate professor in the department of earth sciences at the university.

In that position, the tusk-like teeth could not have been used for biting, he says.

“That was definitely a surprising moment,” says Davis, who serves as director of the Condon Fossil Collection at the university’s Museum of Natural and Cultural History.

“I realized that all of the artwork and all of the publicity materials and bumper stickers and buttons and T-shirts we had just made two months prior, for the new exhibit, were all out of date,” he says with a laugh.

Davis is co-author of the new study in the journal PLOS One, which renames the giant fish the “spike-toothed salmon.”

It says the salmon used the tusk-like spikes for building nests to spawn, and as defence mechanisms against predators and other salmon.

The salmon lived about five million years ago at a time when Earth was transitioning from warmer to relatively cooler conditions, Davis says.

It’s hard to know exactly why the relatives of today’s sockeye went extinct, but Davis says the cooler conditions would have affected the productivity of the Pacific Ocean and the amount of rain feeding rivers that served as their spawning areas.

Another co-author, Brian Sidlauskas, says a fish the size of the spike-toothed salmon must have been targeted by predators such as killer whales or sharks.

“I like to think … it’s almost like a sledgehammer, these salmon swinging their head back and forth in order to fend off things that might want to feast on them,” he says.

Sidlauskas says analysis by the lead author of the paper, Kerin Claeson, found both male and female salmon had the “multi-functional” spike-tooth feature.

“That’s part of our reason for hypothesizing that this tooth is multi-functional … It could easily be for digging out nests,” he says.

“Think about how big the (nest) would have to be for an animal of this size, and then carving it out in what’s probably pretty shallow water; and so having an extra digging tool attached to your head could be really useful.”

Sidlauskas says the giant salmon help researchers understand the boundaries of what’s possible with the evolution of salmon, but they also capture the human imagination and a sense of wonder about what’s possible on Earth.

“I think it helps us value a little more what we do still have, or I hope that it does. That animal is no longer with us, but it is a product of the same biosphere that sustains us.”

This report by The Canadian Press was first published April 24, 2024.

Brenna Owen, The Canadian Press

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