In a pair of world firsts, UBC scientists have 3D printed human testicular cells and identified promising early signs of sperm-producing capabilities.
The researchers, led by UBC urology assistant professor Dr. Ryan Flannigan, hope the technique will one day offer a solution for people living with presently untreatable forms of male infertility.
“Infertility affects 15 per cent of couples and male factors are a contributing cause in at least half those cases,” said Dr. Flannigan, whose lab is based at the Vancouver Prostate Centre at Vancouver General Hospital.
“We’re 3D printing these cells into a very specific structure that mimics human anatomy, which we think is our best shot at stimulating sperm production. If successful, this could open the door to new fertility treatments for couples who currently have no other options.”
Within human testicles, sperm is produced by tiny tubes known as seminiferous tubules. In the most severe form of male infertility, known as non-obstructive azoospermia (NOA), no sperm is found in ejaculate due to diminished sperm production within these structures.
While in some cases doctors can help NOA patients by performing surgery to find extremely rare sperm, Dr. Flannigan says this procedure is only successful about half the time.
“Unfortunately, for the other half of these individuals, they don’t have any options because we can’t find sperm for them.”
Those are the patients Dr. Flannigan’s team is hoping to help.
For the recent study, the researchers performed a biopsy to collect stem cells from the testicles of a patient living with NOA. The cells were then grown and 3D printed onto a petri dish into a hollow tubular structure that resembles the sperm-producing seminiferous tubules.
Twelve days after printing, the team found that the cells had survived. Not only that, they had matured into several of the specialized cells involved in sperm production and were showing a significant improvement in spermatogonial stem cell maintenance – both early signs of sperm producing capabilities. The results of the study were recently published in Fertility and Sterility Science.
“It’s a huge milestone, seeing these cells survive and begin to differentiate. There’s a long road ahead, but this makes our team very hopeful,” said Dr. Flannigan.
The team is now working to “coach” the printed cells into producing sperm. To do this, they’ll expose the cells to different nutrients and growth factors and fine-tune the structural arrangement to facilitate cell-to-cell interaction.
If they can get the cells to produce sperm, those sperm could potentially be used to fertilize an egg by in vitro fertilization, providing a new fertility treatment option for couples.
Dr. Flannigan’s research program has also been shedding new light on the genetic and molecular mechanisms that contribute to NOA. They’ve been using various single cell sequencing techniques to understand the gene expression and characteristics of each individual cell, then applying computational modelling of this data to better understand the root causes of the condition and to identify new treatment options. The work has been highly collaborative, involving UBC researchers across computer science, mathematics and engineering, as well as international collaborations.
“Increasingly, we’re learning that there are likely many different causes of infertility and that each case is very patient specific,” said Dr. Flannigan. “With that in mind, we’re taking a personalized, precision medicine approach – we take cells from a patient, try to understand what abnormalities are unique to them, and then 3D print and support the cells in ways that overcome those original deficiencies.”
Method of Research
Subject of Research
Using Clinically Derived Human Tissue to 3D Bioprint Personalized Testicular Tubules For in-Vitro Culturing. First Report
Article Publication Date
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Green comet making its closest approach to Earth in 50,000 years – Yahoo Movies Canada
A rare green comet, that has not been seen for 50,000 years, is about to make its closest pass by Earth, becoming visible in a once-in-a-lifetime opportunity.
Called C/2022 E3 (ZTF), this celestial object hails from the Oort cloud at the outermost edge of the solar system.
Its green glow is a result of ultraviolet radiation from the sun lighting up the gases surrounding the comet’s surface.
The icy ball orbits the sun once every 50,000 years, which means the last time it went past the planet was during the Stone Age – when Neanderthals roamed the Earth.
It is due to pass closest to the planet – still some 42 million kilometres away – on Wednesday night, into the early hours of Thursday and in a very dark sky will appear as a faint smudge to those looking for it with the naked eye.
However, even if the moon is too bright for stargazers to spot the comet on Wednesday night, they might be able to catch a glimpse of it a week later when it passes Mars.
Professor Don Pollacco, from the department of physics at the University of Warwick, told the PA news agency: “Comet C/2022 E3 passes closest to Earth tonight, on 1 February.
“It has been christened the “Green Comet” as pictures show the head of the Comet to have a striking colour.
“We understand this as due to light emitted from carbon molecules ejected from the nucleus due to the increase in heat etc during its closest approach to the sun, which happened around 12 January.
“Some comets approach the sun much closer and are completely evaporated by the intense radiation.”
He added: “As the comet approaches Earth (it’s still 42 million km away, so no chance of a collision) it appears to move more quickly across the sky on a night-by-night basis.
“Tonight the comet is about halfway between the pole star and the bright star Capella, overhead about 11pm.
“However, the waxing moon will make the Comet much harder to spot. To see it you’ll need a clear sky, binoculars and a bit of luck.
“Alternately, if you wait a few days to around 10 February, the moon will be less bright and the comet will be clearer to see in the southern part of the sky, passing Mars.”
The Greenwich Royal Observatory says that from the northern hemisphere, the comet is already visible in the night sky using a telescope or some binoculars.
It adds: “Comet C/2022 E3 (ZTF) will be closest to Earth on February 1. This will also be the moment the comet appears at its brightest, and currently it is expected to reach a brightness magnitude of +6. That would mean it would be visible to the naked eye.
“It’s worth noting, however, that comets can be unpredictable, and it’s hard to say with accuracy how bright the comet will be or what it will look like ahead of time.
“The comet looks like a fuzzy green ball or smudge in the sky. This green glow is a result of UV radiation from the sun lighting up the gases streaming off of the comet’s surface.”
Advising on where the comet can be seen in the night sky, the Observatory says: “When it passes near Earth in February, the green comet will be in the constellation of Camelopardalis.
“After its closest approach, the green comet will move through Auriga and end up in Taurus mid-February.
“The comet will dim over the month as it moves away from us, and the time that it will be up in the sky during the night will get shorter and shorter.”
New AI algorithm helps find 8 radio signals from space
A new artificial intelligence algorithm created by a Toronto student is helping researchers search the stars for signs of life.
Peter Xiangyuan Ma, a University of Toronto undergraduate student and researcher, said he started working on the algorithm while he was in Grade 12 during the pandemic.
“I was just looking for projects and I was interested in astronomy,” he told CTV News Toronto.
The idea was to help distinguish between technological radio signals created by human technologies and signals that were potentially coming from other forms of life in space.
“What we’re looking for is signs of technology that signifies if the sender is intelligent or not. And so unsurprised to us, we keep on finding ourselves,” Ma explained. “We don’t want to be looking at our own noisy signals.”
Using this algorithm, Ma said researchers were able to discover eight new radio signals being emitted from five different stars about 30 to 90 light years away from the Earth.
These signals, Ma said, would disappear when researchers looked away from it, which rules out, for the most part, interference from a signal originating from Earth. When they returned to the area, the signal was still there.
“We’re all very suspicious and scratching our heads,” he said. “We proved that we found things that we wanted to find … now, what do we do with all these? That’s another separate issue.”
Steve Croft, Project Scientist for Breakthrough Listen on the Green Bank Telescope, the institute whose open source data was the inspiration for Ma’s algorithm, said that finding radio signals in space is like trying to find a needle in a haystack.
“You’ve got to recognize the haystack itself and make sure that you don’t throw the needle away as you’re looking at the individual pieces of hay,” Croft, who collaborated on Ma’s research, told CTV News Toronto.
Croft said algorithms being used to discover these signals have to account for multiple characteristics, including the position they are coming from in the sky and whether or not the transmission changes over time, which could indicate if it’s coming from a rotating planet or star.
“The algorithm that Peter developed has enabled us to do this more efficiently,” he said.
The challenge, Croft says, is recognizing that false positives may exist despite a signal meeting this criteria. What could be signs of extraterrestrial life may also just be a “weirdly shaped bit of a haystack,” he added.
“And so that’s why we have to go back and look again and see if the signal still there. And with these particular examples that Peter found with his algorithm, the signal was not there when we pointed the telescope back again. And so we sort of can’t say one way or another, is this genuine?”
Researchers have been searching the sky for technologically-generated signals since the 1960s, searching thousands of stars and galaxies for signs of intelligent life. The process is called “SETI,” or “the Search for Extraterrestrial Intelligence.”
But interference from our own radio signals has always proven to be a challenge. Croft says most pieces of technology have some kind of Bluetooth or wireless wave element that creates static, resulting in larger amounts of data needed to be collected.
“That’s a challenge but also computing provides the solution,” he said.
“So the computing and particularly the machine-learning algorithms gives us the power to search through this big haystack, looking for the needle of an interesting signal.”
Ma said that while we may not have found a “technosignal” just yet, we shouldn’t give up. The next step would be to employ multiple kinds of search algorithms to find more and more signals to study.
While the “dream” is to find evidence of life, Ma says he is more focused on the scientific efforts of actively looking for it.
This sentiment is echoed by Croft, who said he is most fascinating in working towards answering the question of whether humans are alone in this universe.
“I don’t show up to work every day, thinking I’m going to find aliens, but I do show up for work. So you know, I’ve got sort of some optimism.”
How to spot the green comet in Manitoba
Space enthusiasts in the province will get the chance to potentially see a rare green comet over the next couple of days.
The comet was discovered by astronomers in southern California last year and it was determined the last time it passed Earth was around 50,000 years ago.
Mike Jensen, the planetarium and science gallery program supervisor at the Manitoba Museum, said the time between appearances and the colour of the comet makes this unique compared to others.
“The last time it would have appeared anywhere within the region of visibility to Earth, we’re talking primitive humans walking the Earth,” said Jensen. “And then yes, its colour. Most people associate comets, they’re often referred to as ghosts of the night sky because they often have a bit of a whitish-blue appearance. This one’s got a bit of green to it. Comets are all made up of different types of material, this just happens to have a bit more of some carbon elements in it.”
Jensen notes the green tint on the comet will be subtle, comparing it to the subtle red that surrounds Mars in the night sky.
Wednesday and Thursday are the best days to see the comet as Jensen said that’s when it will be closest to Earth – 42 million kilometres away.
“That proximity to us means it does get to its best visibility for us. The added advantage is it’s also appearing sort of high up in the northern sky, which puts it amongst the circumpolar stars of our night sky. In other words, the stars that are circling around the North Star.”
Now, just because the comet is close enough to be visible doesn’t mean it will be the easiest to see in the night sky according to Jensen. He said there are a few factors that play into having a successful sighting.
First, he suggests getting out of the city and away from the lights, noting, the darker it is, the better. If people head outside city limits, Jensen recommends people dress warmly, saying comet watching in the winter is not for the “faint of heart.”
Secondly, he said even though it might be possible to see the comet with the naked eye, he still suggests bringing binoculars to improve people’s chances. He also recommends checking star maps before leaving to get the most accurate location of where the comet may be.
Lastly, even if all of that is achieved, Jensen notes people will have to battle with the light of the moon, as it is close to a full moon.
“I’m not trying to dissuade anybody from going out to see it, but certainly, there’s going to be some hurdles to overcome in order to be able to spot it on your own.”
If people don’t want to go outside to see it, he said there are plenty of resources online to find digital views.
– With files from CTV News’ Michael Lee
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